Identification of a diazinon-metabolizing glutathione S-transferase in the silkworm, Bombyx mori

研究成果: Contribution to journalArticle査読

26 被引用数 (Scopus)

抄録

The glutathione S-transferase superfamily play key roles in the metabolism of numerous xenobiotics. We report herein the identification and characterization of a novel glutathione S-transferase in the silkworm, Bombyx mori. The enzyme (bmGSTu2) conjugates glutathione to 1-chloro-2,4-dinitrobenzene, as well as metabolizing diazinon, one of the organophosphate insecticides. Quantitative reverse transcription-polymerase chain reaction analysis of transcripts demonstrated that bmGSTu2 expression was induced 1.7-fold in a resistant strain of B. mori. Mutagenesis of putative amino acid residues in the glutathione-binding site revealed that Ile54, Glu66, Ser67, and Asn68 are crucial for enzymatic function. These results provide insights into the catalysis of glutathione conjugation in silkworm by bmGSTu2 and into the detoxification of organophosphate insecticides.

本文言語英語
論文番号30073
ジャーナルScientific reports
6
DOI
出版ステータス出版済み - 7 21 2016

All Science Journal Classification (ASJC) codes

  • 一般

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