TY - JOUR
T1 - Identification of a Human Clonogenic Progenitor with Strict Monocyte Differentiation Potential
T2 - A Counterpart of Mouse cMoPs
AU - Kawamura, Shunsuke
AU - Onai, Nobuyuki
AU - Miya, Fuyuki
AU - Sato, Taku
AU - Tsunoda, Tatsuhiko
AU - Kurabayashi, Kazutaka
AU - Yotsumoto, Satoshi
AU - Kuroda, Shoko
AU - Takenaka, Katsuto
AU - Akashi, Koichi
AU - Ohteki, Toshiaki
N1 - Funding Information:
We thank H. Kamioka for secretarial support, H. Kawamoto (Kyoto University) for the Dll4+ Tst4 stromal cells, K. Sugimoto (Niigata University) and N. Kiyokawa (National Center for Child Health and Development) for the MS5 stromal cells, the Japanese Red Cross Kanto-Koshinetsu Cord Blood Bank (Tokyo) for umbilical-cord blood, and Kyowa Hakko Kirin for human recombinant thrombopoietin. This work was supported by the Uehara Memorial Foundation (T.O.), the SECOM Science and Technology Foundation (T.O.), a Grant-in-Aid for JSPS Fellows (6043, S. Kawamura), and a Grant-in-Aid for Scientific Research and a Grant-in-Aid for Exploratory Research from the Ministry of Education, Science, Sports and Culture of Japan (24590576 to N.O. and 26253029 and 15K15150 to T.O.).
Publisher Copyright:
© 2017 Elsevier Inc.
PY - 2017/5/16
Y1 - 2017/5/16
N2 - Monocytes give rise to macrophages and dendritic cells (DCs) under steady-state and inflammatory conditions, thereby contributing to host defense and tissue pathology. A common monocyte progenitor (cMoP) that is strictly committed to the monocyte lineage has been recently identified in mice. Here, we identified human cMoPs as a CLEC12AhiCD64hi subpopulation of conventional granulocyte-monocyte progenitors (cGMPs) in umbilical cord blood and in bone marrow. Human cMoPs gave rise to monocyte subsets without showing any potential for differentiating into myeloid or lymphoid cells. Within the cGMP population, we also identified revised GMPs that completely lacked DC and lymphoid potential. Collectively, our findings expand and revise the current understanding of human myeloid cell differentiation pathways.
AB - Monocytes give rise to macrophages and dendritic cells (DCs) under steady-state and inflammatory conditions, thereby contributing to host defense and tissue pathology. A common monocyte progenitor (cMoP) that is strictly committed to the monocyte lineage has been recently identified in mice. Here, we identified human cMoPs as a CLEC12AhiCD64hi subpopulation of conventional granulocyte-monocyte progenitors (cGMPs) in umbilical cord blood and in bone marrow. Human cMoPs gave rise to monocyte subsets without showing any potential for differentiating into myeloid or lymphoid cells. Within the cGMP population, we also identified revised GMPs that completely lacked DC and lymphoid potential. Collectively, our findings expand and revise the current understanding of human myeloid cell differentiation pathways.
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U2 - 10.1016/j.immuni.2017.04.019
DO - 10.1016/j.immuni.2017.04.019
M3 - Article
C2 - 28514689
AN - SCOPUS:85019481421
VL - 46
SP - 835-848.e4
JO - Immunity
JF - Immunity
SN - 1074-7613
IS - 5
ER -
