Identification of a soluble isoform of human IL-17RA generated by alternative splicing

Miwa Sohda, Yoshio Misumi, Kosuke Tashiro, Manabu Yamazaki, Takashi Saku, Kimimitsu Oda

研究成果: Contribution to journalArticle査読

11 被引用数 (Scopus)

抄録

IL-17RA, a member of the interleukin (IL)-17 receptor family, is a single membrane-spanning protein that ubiquitously expressed on the cell surface. IL-17RA transduces IL-17A, IL-17F, and IL-17A/F heterodimer-mediated signals by forming a complex with IL-17RC, and also signals the IL-17E (also known as IL-25) response in combination with IL-17RB (also known as IL-25R). Previously, soluble isoforms of human IL-17RC and IL-17RB have been reported, but the existence of a soluble isoform of human IL-17RA has remained unclear. Here, we report the identification of a soluble isoform of human IL-17RA at the mRNA and protein levels. Reverse transcribed PCR experiments showed that the IL-17RA variant is generated by spliced out of exon 11 encoding the transmembrane region in a variety of human tissues. The soluble IL-17RA isoform was detected in the culture media of human cell lines by Western blotting. The existence of the soluble IL-17RA isoform sheds new light on the regulation of IL-17RA mediated responses.

本文言語英語
ページ(範囲)642-645
ページ数4
ジャーナルCytokine
64
3
DOI
出版ステータス出版済み - 12 2013

All Science Journal Classification (ASJC) codes

  • 免疫アレルギー学
  • 免疫学
  • 生化学
  • 血液学
  • 分子生物学

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