TY - JOUR
T1 - Identification of an hla-peptide complex contributing to japanese cedar pollinosis
AU - Hori, Toshio
AU - Kamikawaji, Nobuhiro
AU - Kimura, Akinori
AU - Sasazuki, Takehiko
PY - 1996
Y1 - 1996
N2 - We investigated the association between Japanese cedar pollinosis and HLA-class II alleles by HLA-DNA typing using a PCR-SSOP method and found that the frequency of HLA-DP5 (DPA 1*02022 and DPB 1*0501) was significantly increased in the patients. To investigate whether the HLA-DP5 was merely a genetic marker or rather involved in the pathogenesis of the disease, Japanese cedar pollen antigen (CPAg)-specific T cell lines were established from three patients who possessed HLA-DR4-DR53-DQ4-DP5 (DRB1 *0405-DRB4*01 -DQA1*0302-DQB1*0401 -DPA1*02022-DPB l *0501) haplotype. By using these CPAg-specific T cell lines and 88 synthesized overlapping peptides spanning the entire length of two major cedar pollen allergens, Cry j 1 and Cry j 2, two peptides recognized by DR53-restricted T cells and one peptide recognized by DPS-restricted T cells were identified. DPS-restricted T cell lines as well as DR53-restricted T cell lines produced Th2-type cytokines. Therefore, HLA-DP5 may be directly involved in the pathogenesis, by helping the IgE Ab production to CPAg.
AB - We investigated the association between Japanese cedar pollinosis and HLA-class II alleles by HLA-DNA typing using a PCR-SSOP method and found that the frequency of HLA-DP5 (DPA 1*02022 and DPB 1*0501) was significantly increased in the patients. To investigate whether the HLA-DP5 was merely a genetic marker or rather involved in the pathogenesis of the disease, Japanese cedar pollen antigen (CPAg)-specific T cell lines were established from three patients who possessed HLA-DR4-DR53-DQ4-DP5 (DRB1 *0405-DRB4*01 -DQA1*0302-DQB1*0401 -DPA1*02022-DPB l *0501) haplotype. By using these CPAg-specific T cell lines and 88 synthesized overlapping peptides spanning the entire length of two major cedar pollen allergens, Cry j 1 and Cry j 2, two peptides recognized by DR53-restricted T cells and one peptide recognized by DPS-restricted T cells were identified. DPS-restricted T cell lines as well as DR53-restricted T cell lines produced Th2-type cytokines. Therefore, HLA-DP5 may be directly involved in the pathogenesis, by helping the IgE Ab production to CPAg.
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M3 - Article
AN - SCOPUS:33748186180
VL - 41
SP - 104
JO - Jinrui idengaku zasshi. The Japanese journal of human genetics
JF - Jinrui idengaku zasshi. The Japanese journal of human genetics
SN - 1434-5161
IS - 1
ER -