Identification of an imprinted U2af binding protein related sequence on mouse chromosome 11 using the RLGS method

Y. Hayashizaki; H. Shibata; S. Hirotsune; H. Sugino; Y. Okazaki; N. Sasaki; K. Hirose; H. Imoto; H. Okuizumi; M. Muramatsu; H. Komatsubara; T. Shiroishi; K. Moriwaki; M. Katsuki; N. Hatano; H. Sasaki; T. Ueda; N. Mise; N. Takagi; C. Plass; V. M. Chapman

doi:10.1038/ng0194-33

Identification of an imprinted U2af binding protein related sequence on mouse chromosome 11 using the RLGS method

Y. Hayashizaki, H. Shibata, S. Hirotsune, H. Sugino, Y. Okazaki, N. Sasaki, K. Hirose, H. Imoto, H. Okuizumi, M. Muramatsu, H. Komatsubara, T. Shiroishi, K. Moriwaki, M. Katsuki, N. Hatano, H. Sasaki, T. Ueda, N. Mise, N. Takagi, C. PlassV. M. Chapman

分子機能制御学部門

研究成果: ジャーナルへの寄稿 › 学術誌 › 査読

30 被引用数 (Scopus)

抄録

A new imprinted gene has been discovered in mice using the technique of restriction landmark genomic scanning (RGLS) with methylation sensitive enzymes. Eight out of 3,100 strain–specific Notl and BssHII spots were identified as imprinted in reciprocal F₁ hybrids. Subsequently, we isolated a genomic clone for one locus on proximal chromosome 11 near the Glns locus, an imprinted region in uniparental disomic mice, and its corresponding cDNA clone. Expression of this transcript from the paternal allele was established using RT–PCR of reciprocal F₁–hybrid mice. The amino–acid sequence deduced from the cDNA showed significant homology to the U2 small nuclear ribonucleoprotein auxiliary factor 35 kDa subunit.

本文言語	英語
ページ（範囲）	33-40
ページ数	8
ジャーナル	Nature genetics
巻	6
号	1
DOI	https://doi.org/10.1038/ng0194-33
出版ステータス	出版済み - 1月 1994

!!!All Science Journal Classification (ASJC) codes

遺伝学

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10.1038/ng0194-33

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Hayashizaki, Y., Shibata, H., Hirotsune, S., Sugino, H., Okazaki, Y., Sasaki, N., Hirose, K., Imoto, H., Okuizumi, H., Muramatsu, M., Komatsubara, H., Shiroishi, T., Moriwaki, K., Katsuki, M., Hatano, N., Sasaki, H., Ueda, T., Mise, N., Takagi, N., ... Chapman, V. M. (1994). Identification of an imprinted U2af binding protein related sequence on mouse chromosome 11 using the RLGS method. Nature genetics, 6(1), 33-40. https://doi.org/10.1038/ng0194-33

Hayashizaki, Y, Shibata, H, Hirotsune, S, Sugino, H, Okazaki, Y, Sasaki, N, Hirose, K, Imoto, H, Okuizumi, H, Muramatsu, M, Komatsubara, H, Shiroishi, T, Moriwaki, K, Katsuki, M, Hatano, N, Sasaki, H, Ueda, T, Mise, N, Takagi, N, Plass, C & Chapman, VM 1994, 'Identification of an imprinted U2af binding protein related sequence on mouse chromosome 11 using the RLGS method', Nature genetics, vol. 6, no. 1, pp. 33-40. https://doi.org/10.1038/ng0194-33

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title = "Identification of an imprinted U2af binding protein related sequence on mouse chromosome 11 using the RLGS method",

abstract = "A new imprinted gene has been discovered in mice using the technique of restriction landmark genomic scanning (RGLS) with methylation sensitive enzymes. Eight out of 3,100 strain–specific Notl and BssHII spots were identified as imprinted in reciprocal F1 hybrids. Subsequently, we isolated a genomic clone for one locus on proximal chromosome 11 near the Glns locus, an imprinted region in uniparental disomic mice, and its corresponding cDNA clone. Expression of this transcript from the paternal allele was established using RT–PCR of reciprocal F1–hybrid mice. The amino–acid sequence deduced from the cDNA showed significant homology to the U2 small nuclear ribonucleoprotein auxiliary factor 35 kDa subunit.",

author = "Y. Hayashizaki and H. Shibata and S. Hirotsune and H. Sugino and Y. Okazaki and N. Sasaki and K. Hirose and H. Imoto and H. Okuizumi and M. Muramatsu and H. Komatsubara and T. Shiroishi and K. Moriwaki and M. Katsuki and N. Hatano and H. Sasaki and T. Ueda and N. Mise and N. Takagi and C. Plass and Chapman, {V. M.}",

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AU - Sugino, H.

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AU - Sasaki, N.

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AU - Imoto, H.

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AU - Muramatsu, M.

AU - Komatsubara, H.

AU - Shiroishi, T.

AU - Moriwaki, K.

AU - Katsuki, M.

AU - Hatano, N.

AU - Sasaki, H.

AU - Ueda, T.

AU - Mise, N.

AU - Takagi, N.

AU - Plass, C.

AU - Chapman, V. M.

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Identification of an imprinted U2af binding protein related sequence on mouse chromosome 11 using the RLGS method

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