Identification of an inhibitor for interleukin 4-induced ε germline transcription and antigen-specific IgE production in vivo

Hirofumi Tachibana, Takao Kubo, Toshio Miyase, Sousuke Tanino, Miki Yoshimoto, Mitsuaki Sano, Mari Yamamoto-Maeda, Koji Yamada

研究成果: Contribution to journalArticle査読

52 被引用数 (Scopus)

抄録

IgE plays a key role in the pathogenesis of allergic disease. Interleukin (IL) 4 is a potent and critical stimulator of immunoglobulin class switching from IgM to IgE in B cells. IL-4 induces the expression of ε germline transcript (εGT), which is critical to initiate IgE production. While searching for molecules that inhibit εGT expression induced by IL-4, we found that polyphenol strictinin, which was isolated from tea leaves, was able to inhibit the IL-4-induced εGT expression in the human B cell line DND39. Strictinin also acted on human peripheral blood mononuclear cells obtained from healthy donors to inhibit IL-4-induced εGT expression. Strictinin demonstrated similar inhibitory activity in peripheral blood mononuclear cells obtained from atopic donors. Interestingly, strictinin decreased ovalbumin-induced IgE production in mice, whereas the production of IgG and IgM was not affected. Furthermore, we found that the IL-4-induced STAT6 tyrosine phosphorylation, which is essential for IL-4-induced εGT expression, was inhibited in DND39 cells upon treatment with strictinin. Taken together, these results suggest that strictinin can inhibit IgE production through the inhibition of IL-4-mediated signaling in B cells.

本文言語英語
ページ(範囲)53-60
ページ数8
ジャーナルBiochemical and Biophysical Research Communications
280
1
DOI
出版ステータス出版済み - 2001

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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