Identification of anaerobic arsenite-oxidizing and arsenate-reducing bacteria associated with an alkaline saline lake in Khovsgol, Mongolia

Natsuko Hamamura, Takaaki Itai, Yitai Liu, Anna Louise Reysenbach, Narantuya Damdinsuren, William P. Inskeep

研究成果: ジャーナルへの寄稿記事

19 引用 (Scopus)

抄録

Microbial arsenic transformation pathways associated with a saline lake located in northern Mongolia were examined using molecular biological and culturing approaches. Bacterial 16S rRNA gene sequences recovered from saline lake sediments and soils were affiliated with haloalkaliphiles, including Bacillus and Halomonas spp. Diverse sequences of arsenate respiratory reductase (arrA) and a new group of arsenite oxidase (arxA) genes were also identified. Pure cultures of arsenate-reducing Nitrincola strain and anaerobic arsenite-oxidizing Halomonas strain were isolated. The chemoorganotrophic Halomonas strain contains arxA gene similar to that of a chemoautotrophic arsenite-oxidizing Alkalilimnicola ehrlichii strain MLHE-1. These results revealed the diversity of arsenic transformation pathways associated with a geographically distinct saline system and the potential contribution of arx-dependent arsenite oxidation by heterotrophic bacteria.

元の言語英語
ページ(範囲)476-482
ページ数7
ジャーナルEnvironmental Microbiology Reports
6
発行部数5
DOI
出版物ステータス出版済み - 10 1 2014
外部発表Yes

Fingerprint

Halomonas
Mongolia
arsenites
arsenates
arsenite
saline lake
arsenate
Lakes
Arsenic
Bacteria
lakes
bacterium
Arsenate Reductases
bacteria
arsenic
gene
rRNA Genes
Bacillus
Genes
Bacillus (bacteria)

All Science Journal Classification (ASJC) codes

  • Ecology, Evolution, Behavior and Systematics
  • Agricultural and Biological Sciences (miscellaneous)

これを引用

Identification of anaerobic arsenite-oxidizing and arsenate-reducing bacteria associated with an alkaline saline lake in Khovsgol, Mongolia. / Hamamura, Natsuko; Itai, Takaaki; Liu, Yitai; Reysenbach, Anna Louise; Damdinsuren, Narantuya; Inskeep, William P.

:: Environmental Microbiology Reports, 巻 6, 番号 5, 01.10.2014, p. 476-482.

研究成果: ジャーナルへの寄稿記事

Hamamura, Natsuko ; Itai, Takaaki ; Liu, Yitai ; Reysenbach, Anna Louise ; Damdinsuren, Narantuya ; Inskeep, William P. / Identification of anaerobic arsenite-oxidizing and arsenate-reducing bacteria associated with an alkaline saline lake in Khovsgol, Mongolia. :: Environmental Microbiology Reports. 2014 ; 巻 6, 番号 5. pp. 476-482.
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abstract = "Microbial arsenic transformation pathways associated with a saline lake located in northern Mongolia were examined using molecular biological and culturing approaches. Bacterial 16S rRNA gene sequences recovered from saline lake sediments and soils were affiliated with haloalkaliphiles, including Bacillus and Halomonas spp. Diverse sequences of arsenate respiratory reductase (arrA) and a new group of arsenite oxidase (arxA) genes were also identified. Pure cultures of arsenate-reducing Nitrincola strain and anaerobic arsenite-oxidizing Halomonas strain were isolated. The chemoorganotrophic Halomonas strain contains arxA gene similar to that of a chemoautotrophic arsenite-oxidizing Alkalilimnicola ehrlichii strain MLHE-1. These results revealed the diversity of arsenic transformation pathways associated with a geographically distinct saline system and the potential contribution of arx-dependent arsenite oxidation by heterotrophic bacteria.",
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AU - Damdinsuren, Narantuya

AU - Inskeep, William P.

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AB - Microbial arsenic transformation pathways associated with a saline lake located in northern Mongolia were examined using molecular biological and culturing approaches. Bacterial 16S rRNA gene sequences recovered from saline lake sediments and soils were affiliated with haloalkaliphiles, including Bacillus and Halomonas spp. Diverse sequences of arsenate respiratory reductase (arrA) and a new group of arsenite oxidase (arxA) genes were also identified. Pure cultures of arsenate-reducing Nitrincola strain and anaerobic arsenite-oxidizing Halomonas strain were isolated. The chemoorganotrophic Halomonas strain contains arxA gene similar to that of a chemoautotrophic arsenite-oxidizing Alkalilimnicola ehrlichii strain MLHE-1. These results revealed the diversity of arsenic transformation pathways associated with a geographically distinct saline system and the potential contribution of arx-dependent arsenite oxidation by heterotrophic bacteria.

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