Identification of Bombyx mori 14-3-3 orthologs and the interactor Hsp60

Hiroko Tabunoki, Toru Shimada, Yutaka Banno, Ryoichi Sato, Hideyuki Kajiwara, Kazuei Mita, Jun ichi Satoh

研究成果: ジャーナルへの寄稿記事

16 引用 (Scopus)

抄録

The 14-3-3 protein family consists of evolutionarily conserved, acidic 30 kDa proteins composed of seven isoforms named β, γ, ε, ζ, η, θ, and σ in mammalian cells. The dimeric complex of 14-3-3 isoforms, acting as a molecular adaptor, plays a central role in regulation of neuronal function. Since aberrant expression of 14-3-3 is identified in the brains of Alzheimer disease and Parkinson disease, a convenient insect model, if it is available, is highly valuable for studying a pathological role of 14-3-3 in neurodegeneration. Here, we identified the silkworm Bombyx mori 14-3-3 orthologs, ζ and ε isoforms highly homologous in amino acid sequences to the human 14-3-3ζ and 14-3-3ε. By Western blot, the expression of ζ and ε isoforms was identified at substantial levels in the first instar larva, markedly upregulated in the second instar larva, and the highest levels were maintained in the late stage of larva, the pupa, and the adult. Furthermore, by protein overlay and immunoprecipitation, we identified Hsp60 as a 14-3-3-binding partner. The 14-3-3 proteins interacted with the N-terminal fragment of Hsp60. The 14-3-3ζ and ε isoforms, along with Hsp60, were expressed widely with overlapping distribution in larval and adult tissues, including brain, fat body, silk gland, Malpighian tube, midgut, ovary, testis, antenna, and pheromone gland. These observations suggest that a molecular adaptor 14-3-3 and a molecular chaperone Hsp60 cooperate to achieve a wide range of cellular functions in B. mori.

元の言語英語
ページ(範囲)271-280
ページ数10
ジャーナルNeuroscience Research
61
発行部数3
DOI
出版物ステータス出版済み - 7 1 2008

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Bombyx
Protein Isoforms
14-3-3 Proteins
Larva
Amino Acid Sequence Homology
Pupa
Molecular Chaperones
Fat Body
Silk
Pheromones
Brain Diseases
Immunoprecipitation
Parkinson Disease
Insects
Testis
Ovary
Alzheimer Disease
Proteins
Western Blotting
Brain

All Science Journal Classification (ASJC) codes

  • Neuroscience(all)

これを引用

Tabunoki, H., Shimada, T., Banno, Y., Sato, R., Kajiwara, H., Mita, K., & Satoh, J. I. (2008). Identification of Bombyx mori 14-3-3 orthologs and the interactor Hsp60. Neuroscience Research, 61(3), 271-280. https://doi.org/10.1016/j.neures.2008.03.007

Identification of Bombyx mori 14-3-3 orthologs and the interactor Hsp60. / Tabunoki, Hiroko; Shimada, Toru; Banno, Yutaka; Sato, Ryoichi; Kajiwara, Hideyuki; Mita, Kazuei; Satoh, Jun ichi.

:: Neuroscience Research, 巻 61, 番号 3, 01.07.2008, p. 271-280.

研究成果: ジャーナルへの寄稿記事

Tabunoki, H, Shimada, T, Banno, Y, Sato, R, Kajiwara, H, Mita, K & Satoh, JI 2008, 'Identification of Bombyx mori 14-3-3 orthologs and the interactor Hsp60', Neuroscience Research, 巻. 61, 番号 3, pp. 271-280. https://doi.org/10.1016/j.neures.2008.03.007
Tabunoki, Hiroko ; Shimada, Toru ; Banno, Yutaka ; Sato, Ryoichi ; Kajiwara, Hideyuki ; Mita, Kazuei ; Satoh, Jun ichi. / Identification of Bombyx mori 14-3-3 orthologs and the interactor Hsp60. :: Neuroscience Research. 2008 ; 巻 61, 番号 3. pp. 271-280.
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