Identification of bottom-fermenting yeast genes expressed during lager beer fermentation

Satoshi Yoshida, Kaori Hashimoto, Emiko Shimada, Tatsuji Ishiguro, Toshiko Minato, Satoru Mizutani, Hiroyuki Yoshimoto, Kosuke Tashiro, Satoru Kuhara, Osamu Kobayashi

研究成果: ジャーナルへの寄稿学術誌査読

20 被引用数 (Scopus)

抄録

It has been proposed that bottom-fermenting yeast strains of Saccharomyces pastorianus possess at least two types of genomes. Sequences of genes of one genome [S. cerevisiae (Sc)-type] have been found to be highly homologous (more than 90% identity) to S. cerevisiae S288C sequences, while those of the other [Lager (Lg)-type] are less so. To identify and discriminate Lg-type from Sc-type genes expressed during lager beer fermentation, normalized cDNA libraries were constructed and analysed. From approximately 22 000 ESTs, 3892 Sc-type and 2695 Lg-type ORFs were identified. Expression patterns of Sc- and Lg-type genes did not correlate with particular cell functions in KEGG classification system. Moreover, 405 independent clones were isolated that have no significant homology with sequences in the S288C database, suggesting that they include the bottom-fermenting yeast-specific (BFY) genes. Most of BFY genes have significant homology with the S. bayanus genome. The DNA sequences of S. pastorianus non-Sc-type ORFs were submitted to DDBJ/ GenBank/EMBL under Accession Nos CJ992285-CJ998763.

本文言語英語
ページ(範囲)599-606
ページ数8
ジャーナルYeast
24
7
DOI
出版ステータス出版済み - 7月 1 2007

!!!All Science Journal Classification (ASJC) codes

  • バイオテクノロジー
  • バイオエンジニアリング
  • 生化学
  • 応用微生物学とバイオテクノロジー
  • 遺伝学

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