Identification of cDNA sequences encoding the complement components of Zebrafish (Danio rerio)

Vo Kha Tam, Masakazu Tsujikura, Tomonori Somamoto, Miki Nakao

研究成果: ジャーナルへの寄稿記事

6 引用 (Scopus)

抄録

Zebrafish can be utilized for immunological research as a fish model that allows gene targeting, but lacks enough molecular and sequence information on the complement system, a humoral innate immune factor. The present study aimed at identification of all the major complement components at the molecular level with phylogenetic evidence. Complementary DNA sequences encoding Clq, Clr/s, C2 (B/C2-B), C3, C4, C5, C6, C7, C8, C9, factor B (B/C2-A), the mannose-binding lectin (MBL), two MBL-associated serine proteases, properdin, and factor I were identified by cloning from the hepatopancreas and by database mining. As reported for carp and trout, the presence of two distinct isotypes were confirmed for C3, C4, and C7 also in zebrafish, indicating the ancient evolutionary origin of their diversifications in the vertebrate lineage.

元の言語英語
ページ(範囲)373-387
ページ数15
ジャーナルJournal of the Faculty of Agriculture, Kyushu University
54
発行部数2
出版物ステータス出版済み - 10 1 2009

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Mannose-Binding Lectin
Zebrafish
mannose
Danio rerio
lectins
complement
Complementary DNA
Properdin
Hepatopancreas
complementary DNA
Complement Factor B
Gene Targeting
Carps
Trout
Immunologic Factors
hepatopancreas
Serine Proteases
gene targeting
serine proteinases
carp

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Agronomy and Crop Science

これを引用

Identification of cDNA sequences encoding the complement components of Zebrafish (Danio rerio). / Tam, Vo Kha; Tsujikura, Masakazu; Somamoto, Tomonori; Nakao, Miki.

:: Journal of the Faculty of Agriculture, Kyushu University, 巻 54, 番号 2, 01.10.2009, p. 373-387.

研究成果: ジャーナルへの寄稿記事

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