Identification of characteristic compounds of moderate volatility in breast cancer cell lines

Mitsuru Tanaka, Chung Hsuan, Masataka Oeki, Weilin Shen, Asuka Goda, Yusuke Tahara, Takeshi Onodera, Keisuke Sanematsu, Tomotsugu Rikitake, Eiji Oki, Yuzo Ninomiya, Rintaro Kurebayashi, Hideto Sonoda, Yoshihiko Maehara, Kiyoshi Toko, Toshiro Matsui

研究成果: Contribution to journalArticle査読

抄録

In this study, we were challenging to identify characteristic compounds in breast cancer cell lines. GC analysis of extracts from the culture media of breast cancer cell lines (MCF-7, SKBR-3, and YMB-1) using a solid-phase Porapak Q extraction revealed that two compounds of moderate volatility, 1-hexadecanol and 5-(Z)-dodecenoic acid, were detected with markedly higher amount than those in the medium of fibroblast cell line (KMST-6). Furthermore, LC-TOF/MS analysis of the extracts clarified that in addition to the above two fatty acids, the amounts of five unsaturated fatty acids [decenoic acid (C10:1), decadienoic acid (C10:2), 5-(Z)-dodecenoic acid (C12:1), 5-(Z)-Tetradecenoic acid (C14:1), and tetradecadienoic acid (C14:2)] in MCF-7 medium were higher than those in medium of KMST-6. Interestingly, H2O2-oxidation of 5-(Z)-dodecenoic acid and 5-(Z)-Tetradecenoic acid produced volatile aldehydes that were reported as specific volatiles in breath from various cancer patients, such as heptanal, octanal, nonanal, decanal, 2-(E)-nonenal, and 2-(E)-octenal. Thus, we concluded that these identified compounds over-produced in breast cancer cells in this study could serve as potential precursors producing reported cancer-specific volatiles.

本文言語英語
論文番号e0235442
ジャーナルPloS one
15
6 June
DOI
出版ステータス出版済み - 6 2020

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)
  • General

フィンガープリント 「Identification of characteristic compounds of moderate volatility in breast cancer cell lines」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。

引用スタイル