Identification of distinct ligands for the C-type lectin receptors mincle and dectin-2 in the pathogenic fungus Malassezia

Tetsuaki Ishikawa, Fumie Itoh, Sayumi Yoshida, Shinobu Saijo, Tetsuhiro Matsuzawa, Tohru Gonoi, Takashi Saito, Yoshio Okawa, Nobuyuki Shibata, Tomofumi Miyamoto, Sho Yamasaki

研究成果: ジャーナルへの寄稿記事

101 引用 (Scopus)

抄録

Various C-type lectin receptors (CLRs), including Mincle and Dectin-2, function as pattern recognition receptors and play a central role in immunity to fungal pathogens. However, the precise structures of the CLR ligands in various pathogenic fungi have yet to be completely defined. Here we report that Malassezia, an opportunistic skin fungal pathogen, is cooperatively recognized by Mincle and Dectin-2 through distinct ligands. Solvent-based fractionation revealed that Mincle and Dectin-2 recognize lipophilic and hydrophilic components of Malassezia, respectively. Mass spectrometry and nuclear magnetic resonance (NMR) revealed glyceroglycolipid and unique mannosyl fatty acids linked to mannitol as two Mincle ligands. An O-linked mannobiose-rich glycoprotein was identified as a Malassezia ligand for Dectin-2. Cytokine production in response to the Mincle ligands and the Dectin-2 ligand was abrogated in Mincle-/- and Dectin-2-/- dendritic cells, respectively. These results demonstrate that Mincle and Dectin-2 recognize distinct ligands in Malassezia to induce host immune responses.

元の言語英語
ページ(範囲)477-488
ページ数12
ジャーナルCell Host and Microbe
13
発行部数4
DOI
出版物ステータス出版済み - 4 17 2013

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Malassezia
C-Type Lectins
Fungi
Ligands
Pattern Recognition Receptors
Mannitol
mouse dectin-2
Dendritic Cells
Immunity
Mass Spectrometry
Glycoproteins
Magnetic Resonance Spectroscopy
Fatty Acids
Cytokines
Skin

All Science Journal Classification (ASJC) codes

  • Parasitology
  • Microbiology
  • Virology

これを引用

Identification of distinct ligands for the C-type lectin receptors mincle and dectin-2 in the pathogenic fungus Malassezia. / Ishikawa, Tetsuaki; Itoh, Fumie; Yoshida, Sayumi; Saijo, Shinobu; Matsuzawa, Tetsuhiro; Gonoi, Tohru; Saito, Takashi; Okawa, Yoshio; Shibata, Nobuyuki; Miyamoto, Tomofumi; Yamasaki, Sho.

:: Cell Host and Microbe, 巻 13, 番号 4, 17.04.2013, p. 477-488.

研究成果: ジャーナルへの寄稿記事

Ishikawa, T, Itoh, F, Yoshida, S, Saijo, S, Matsuzawa, T, Gonoi, T, Saito, T, Okawa, Y, Shibata, N, Miyamoto, T & Yamasaki, S 2013, 'Identification of distinct ligands for the C-type lectin receptors mincle and dectin-2 in the pathogenic fungus Malassezia', Cell Host and Microbe, 巻. 13, 番号 4, pp. 477-488. https://doi.org/10.1016/j.chom.2013.03.008
Ishikawa, Tetsuaki ; Itoh, Fumie ; Yoshida, Sayumi ; Saijo, Shinobu ; Matsuzawa, Tetsuhiro ; Gonoi, Tohru ; Saito, Takashi ; Okawa, Yoshio ; Shibata, Nobuyuki ; Miyamoto, Tomofumi ; Yamasaki, Sho. / Identification of distinct ligands for the C-type lectin receptors mincle and dectin-2 in the pathogenic fungus Malassezia. :: Cell Host and Microbe. 2013 ; 巻 13, 番号 4. pp. 477-488.
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