Identification of genes differentially regulated in rat alveolar bone wound healing by subtractive hybridization

T. Ohira, F. Myokai, N. Shiomi, K. Yamashiro, T. Yamamoto, Y. Murayama, H. Arai, F. Nishimura, S. Takashiba

研究成果: Contribution to journalArticle

4 引用 (Scopus)

抜粋

Periodontal healing requires the participation of regulatory molecules, cells, and scaffold or matrix. Here, we hypothesized that a certain set of genes is expressed in alveolar bone wound healing. Reciprocal subtraction gave 400 clones from the injured alveolar bone of Wistar rats. Identification of 34 genes and analysis of their expression in injured tissue revealed several clusters of unique gene regulation patterns, including the up-regulation at 1 wk of cytochrome c oxidase regulating electron transfer and energy metabolism, presumably occurring at the site of inflammation; up-regulation at 2.5 wks of pro-α-2 type I collagen involving the formation of a connective tissue structure; and up-regulation at 1 and 2 wks and down-regulation at 2.5 and 4 wks of ubiquitin carboxyl-terminal hydrolase 13 involving cell cycle, DNA repair, and stress response. The differential expression of genes may be associated with the processes of inflammation, wound contraction, and formation of a connective tissue structure.

元の言語英語
ページ(範囲)546-551
ページ数6
ジャーナルJournal of Dental Research
83
発行部数7
DOI
出版物ステータス出版済み - 7 2004
外部発表Yes

All Science Journal Classification (ASJC) codes

  • Dentistry(all)

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    Ohira, T., Myokai, F., Shiomi, N., Yamashiro, K., Yamamoto, T., Murayama, Y., Arai, H., Nishimura, F., & Takashiba, S. (2004). Identification of genes differentially regulated in rat alveolar bone wound healing by subtractive hybridization. Journal of Dental Research, 83(7), 546-551. https://doi.org/10.1177/154405910408300707