Identification of immune-related genes in gill cells of Japanese eels (Anguilla japonica) in adaptation to water salinity changes

Jie Gu, Shuya Dai, Haitao Liu, Quanquan Cao, Shaowu Yin, Keng Po Lai, Ka Fai William Tse, Chris Kong Chu Wong, Haifeng Shi

研究成果: ジャーナルへの寄稿記事

1 引用 (Scopus)

抄録

The changes in ambient salinity influence ion and water homeostasis, hormones secretion, and immune response in fish gills. The physiological functions of hormones and ion transporters in the regulation of gill-osmoregulation have been widely studied, however the modulation of immune response under salinity changes is not determined. Using transcriptome sequencing, we obtained a comprehensive profile of osmo-responsive genes in gill cells of Japanese eel (Anguilla japonica). Herein, we applied bioinformatics analysis to identify the immune-related genes that were significantly higher expressed in gill pavement cells (PVCs) and mitochondrial-rich cells (MRCs) in freshwater (FW) than seawater (SW) adapted fish. We validated the data using the real-time qPCR, which showed a high correlation between the RNA-seq and real-time qPCR data. In addition, the immunohistochemistry results confirmed the changes of the expression of selected immune-related genes, including C-reactive protein (CRP) in PVCs, toll-like receptor 2 (TLR2) in MRCs and interleukin-1 receptor type 2 (IL-1R2) in both PVCs and MRCs. Collectively our results demonstrated that those immune-related genes respond to salinity changes, and might trigger related special signaling pathways and network. This study provides new insights into the impacts of ambient salinity changes on adaptive immune response in fish gill cells.

元の言語英語
ページ(範囲)288-296
ページ数9
ジャーナルFish and Shellfish Immunology
73
DOI
出版物ステータス出版済み - 2 1 2018

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Anguilla japonica
water salinity
eel
gills
Genes
immune response
pavement
Pavements
Fish
salinity
Water
gene
hormone
Interleukin-1 Type II Receptors
genes
fish
Hormones
cells
Ions
Toll-Like Receptor 2

All Science Journal Classification (ASJC) codes

  • Environmental Chemistry
  • Aquatic Science

これを引用

Identification of immune-related genes in gill cells of Japanese eels (Anguilla japonica) in adaptation to water salinity changes. / Gu, Jie; Dai, Shuya; Liu, Haitao; Cao, Quanquan; Yin, Shaowu; Lai, Keng Po; Tse, Ka Fai William; Wong, Chris Kong Chu; Shi, Haifeng.

:: Fish and Shellfish Immunology, 巻 73, 01.02.2018, p. 288-296.

研究成果: ジャーナルへの寄稿記事

Gu, Jie ; Dai, Shuya ; Liu, Haitao ; Cao, Quanquan ; Yin, Shaowu ; Lai, Keng Po ; Tse, Ka Fai William ; Wong, Chris Kong Chu ; Shi, Haifeng. / Identification of immune-related genes in gill cells of Japanese eels (Anguilla japonica) in adaptation to water salinity changes. :: Fish and Shellfish Immunology. 2018 ; 巻 73. pp. 288-296.
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abstract = "The changes in ambient salinity influence ion and water homeostasis, hormones secretion, and immune response in fish gills. The physiological functions of hormones and ion transporters in the regulation of gill-osmoregulation have been widely studied, however the modulation of immune response under salinity changes is not determined. Using transcriptome sequencing, we obtained a comprehensive profile of osmo-responsive genes in gill cells of Japanese eel (Anguilla japonica). Herein, we applied bioinformatics analysis to identify the immune-related genes that were significantly higher expressed in gill pavement cells (PVCs) and mitochondrial-rich cells (MRCs) in freshwater (FW) than seawater (SW) adapted fish. We validated the data using the real-time qPCR, which showed a high correlation between the RNA-seq and real-time qPCR data. In addition, the immunohistochemistry results confirmed the changes of the expression of selected immune-related genes, including C-reactive protein (CRP) in PVCs, toll-like receptor 2 (TLR2) in MRCs and interleukin-1 receptor type 2 (IL-1R2) in both PVCs and MRCs. Collectively our results demonstrated that those immune-related genes respond to salinity changes, and might trigger related special signaling pathways and network. This study provides new insights into the impacts of ambient salinity changes on adaptive immune response in fish gill cells.",
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