Identification of intracellular squalene in living algae, Aurantiochytrium mangrovei with hyper-spectral coherent anti-Stokes Raman microscopy using a sub-nanosecond supercontinuum laser source

Kei Ishitsuka, Masahiro Koide, Masaki Yoshida, Hiroki Segawa, Philippe Leproux, Vincent Couderc, Makoto M. Watanabe, Hideaki Kano

研究成果: Contribution to journalArticle査読

11 被引用数 (Scopus)

抄録

We applied hyper-spectral coherent anti-Stokes Raman scattering imaging to intracellular lipid identification in living microalgae, Aurantiochytrium mangrovei 18W-13a. Two different lipids, squalene and triacylglycerol, were found inside living cells with clear vibrational contrast. Based on the endogenous lipid band as a result of the cis CC stretch vibrational mode, squalene and triacylglycerol were clearly distinguished in different intracellular areas. In particular, squalene was detected solely in vacuoles as lipid particles, which was also supported by electron microscopy.

本文言語英語
ページ(範囲)8-15
ページ数8
ジャーナルJournal of Raman Spectroscopy
48
1
DOI
出版ステータス出版済み - 1 1 2017
外部発表はい

All Science Journal Classification (ASJC) codes

  • 材料科学(全般)
  • 分光学

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