TY - JOUR
T1 - Identification of peptides in blood following oral administration of β-conglycinin to Wistar rats
AU - Sheng, Xiaojing
AU - Nagaoka, Satoshi
AU - Hashimoto, Marika
AU - Amiya, Yusuke
AU - Beppu, Masato
AU - Tsukamoto, Kenichiro
AU - Yanase, Emiko
AU - Tanaka, Mitsuru
AU - Matsui, Toshiro
N1 - Funding Information:
This study was supported in part by Fuji Foundation for Protein Research (2016–2018 to S.N.) and in part by the Grant-in-Aid for the Ministry of Education, Science, Sports, and Culture of Japan (A) awarded to S.N. (No. 17H00817).
Publisher Copyright:
© 2020 Elsevier Ltd
PY - 2021/3/30
Y1 - 2021/3/30
N2 - In this study, β-conglycinin (100 mg/kg) was orally administered to Wistar rats in order to identify peptides that may be derived from the protein in the blood. Plasma samples taken from the tail vein up to 8 h after administration were analyzed by matrix-assisted laser desorption/ionization (MALDI) and liquid chromatography-time-of-flight (LC-TOF) mass spectrometry (MS). In total, 126 signals were detected by MALDI-MS. Among the signals, nine oligopeptides (SEL, KGPL, SILGA, DSEL, GDANI, SYFV, CLQSC, GEQPRPF, and LVINEGDA) were successfully identified as β-conglycinin-derived peptides by LC-TOF/MS at a plasma concentration of 0.75–756 pmol/mL. The results demonstrated that β-conglycinin could be the dietary source protein for the oligopeptides produced prior to entering the circulating bloodstream of rats.
AB - In this study, β-conglycinin (100 mg/kg) was orally administered to Wistar rats in order to identify peptides that may be derived from the protein in the blood. Plasma samples taken from the tail vein up to 8 h after administration were analyzed by matrix-assisted laser desorption/ionization (MALDI) and liquid chromatography-time-of-flight (LC-TOF) mass spectrometry (MS). In total, 126 signals were detected by MALDI-MS. Among the signals, nine oligopeptides (SEL, KGPL, SILGA, DSEL, GDANI, SYFV, CLQSC, GEQPRPF, and LVINEGDA) were successfully identified as β-conglycinin-derived peptides by LC-TOF/MS at a plasma concentration of 0.75–756 pmol/mL. The results demonstrated that β-conglycinin could be the dietary source protein for the oligopeptides produced prior to entering the circulating bloodstream of rats.
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U2 - 10.1016/j.foodchem.2020.128197
DO - 10.1016/j.foodchem.2020.128197
M3 - Article
C2 - 33032251
AN - SCOPUS:85092087064
VL - 341
JO - Food Chemistry
JF - Food Chemistry
SN - 0308-8146
M1 - 128197
ER -