Identification of Quantitative Trait Loci for Resistance to RSIVD in Red Sea Bream (Pagrus major)

Eitaro Sawayama, Shiho Tanizawa, Shin Ichi Kitamura, Kei Nakayama, Kohei Ohta, Akiyuki Ozaki, Motohiro Takagi

研究成果: ジャーナルへの寄稿記事

7 引用 (Scopus)

抄録

Red sea bream iridoviral disease (RSIVD) is a major viral disease in red sea bream farming in Japan. Previously, we identified one candidate male individual of red sea bream that was significantly associated with convalescent individuals after RSIVD. The purpose of this study is to identify the quantitative trait loci (QTL) linked to the RSIVD-resistant trait for future marker-assisted selection (MAS). Two test families were developed using the candidate male in 2014 (Fam-2014) and 2015 (Fam-2015). These test families were challenged with RSIV, and phenotypes were evaluated. Then, de novo genome sequences of red sea bream were obtained through next-generation sequencing, and microsatellite markers were searched and selected for linkage map construction. One immune-related gene, MHC class IIβ, was also used for linkage map construction. Of the microsatellite markers searched, 148 and 197 were mapped on 23 and 27 linkage groups in the female and male linkage maps, respectively, covering approximately 65% of genomes in both sexes. One QTL linked to an RSIVD-resistant trait was found in linkage group 2 of the candidate male in Fam-2014, and the phenotypic variance of the QTL was 31.1%. The QTL was closely linked to MHC class IIβ. Moreover, the QTL observed in Fam-2014 was also significantly linked to an RSIVD-resistant trait in the candidate male of Fam-2015. Our results suggest that the RSIVD-resistant trait in the candidate male was controlled by one major QTL closely linked to the MHC class IIβ gene and could be useful for MAS of red sea bream.

元の言語英語
ページ(範囲)601-613
ページ数13
ジャーナルMarine Biotechnology
19
発行部数6
DOI
出版物ステータス出版済み - 12 1 2017

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Sea Bream
Indian Ocean
Pagrus major
Quantitative Trait Loci
quantitative trait loci
chromosome mapping
MHC Class II Genes
linkage groups
marker-assisted selection
Microsatellite Repeats
genome
sea
Genome
microsatellite repeats
viral disease
gene
Virus Diseases
Agriculture
phenotypic variation
phenotype

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Aquatic Science

これを引用

Identification of Quantitative Trait Loci for Resistance to RSIVD in Red Sea Bream (Pagrus major). / Sawayama, Eitaro; Tanizawa, Shiho; Kitamura, Shin Ichi; Nakayama, Kei; Ohta, Kohei; Ozaki, Akiyuki; Takagi, Motohiro.

:: Marine Biotechnology, 巻 19, 番号 6, 01.12.2017, p. 601-613.

研究成果: ジャーナルへの寄稿記事

Sawayama, E, Tanizawa, S, Kitamura, SI, Nakayama, K, Ohta, K, Ozaki, A & Takagi, M 2017, 'Identification of Quantitative Trait Loci for Resistance to RSIVD in Red Sea Bream (Pagrus major)', Marine Biotechnology, 巻. 19, 番号 6, pp. 601-613. https://doi.org/10.1007/s10126-017-9779-z
Sawayama, Eitaro ; Tanizawa, Shiho ; Kitamura, Shin Ichi ; Nakayama, Kei ; Ohta, Kohei ; Ozaki, Akiyuki ; Takagi, Motohiro. / Identification of Quantitative Trait Loci for Resistance to RSIVD in Red Sea Bream (Pagrus major). :: Marine Biotechnology. 2017 ; 巻 19, 番号 6. pp. 601-613.
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AU - Sawayama, Eitaro

AU - Tanizawa, Shiho

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AU - Nakayama, Kei

AU - Ohta, Kohei

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AB - Red sea bream iridoviral disease (RSIVD) is a major viral disease in red sea bream farming in Japan. Previously, we identified one candidate male individual of red sea bream that was significantly associated with convalescent individuals after RSIVD. The purpose of this study is to identify the quantitative trait loci (QTL) linked to the RSIVD-resistant trait for future marker-assisted selection (MAS). Two test families were developed using the candidate male in 2014 (Fam-2014) and 2015 (Fam-2015). These test families were challenged with RSIV, and phenotypes were evaluated. Then, de novo genome sequences of red sea bream were obtained through next-generation sequencing, and microsatellite markers were searched and selected for linkage map construction. One immune-related gene, MHC class IIβ, was also used for linkage map construction. Of the microsatellite markers searched, 148 and 197 were mapped on 23 and 27 linkage groups in the female and male linkage maps, respectively, covering approximately 65% of genomes in both sexes. One QTL linked to an RSIVD-resistant trait was found in linkage group 2 of the candidate male in Fam-2014, and the phenotypic variance of the QTL was 31.1%. The QTL was closely linked to MHC class IIβ. Moreover, the QTL observed in Fam-2014 was also significantly linked to an RSIVD-resistant trait in the candidate male of Fam-2015. Our results suggest that the RSIVD-resistant trait in the candidate male was controlled by one major QTL closely linked to the MHC class IIβ gene and could be useful for MAS of red sea bream.

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