Identification of reference genes for quantitative PCR analyses in developing mouse gonads

Toshifumi Yokoyama, Takuya Omotehara, Tetsushi Hirano, Naoto Kubota, Shogo Yanai, Chinatsu Hasegawa, Tadashi Takada, Yohei Mantani, Nobuhiko Hoshi

研究成果: ジャーナルへの寄稿コメント/討論査読

6 被引用数 (Scopus)

抄録

Stable reference genes are important for gene expression analyses such as quantitative PCR. The stability of 15 candidate reference genes that can be used to developing mouse gonads was thoroughly verified using combinations of multiple algorithms. The expression of these genes fluctuated greatly depending on the analysis period and/or gender. Peptidylprolyl isomerase A (Ppia) and polymerase (RNA) II (DNA directed) polypeptide A (Polr2a) were the reference genes that were used stably for a wide analysis period in developing mouse gonads. Furthermore, the stable reference genes corresponding to the analysis period and/or gender were ranked. These results are useful for the selection of the optimal reference gene required for high-precision measurements.

本文言語英語
ページ(範囲)1534-1539
ページ数6
ジャーナルJournal of Veterinary Medical Science
80
10
DOI
出版ステータス出版済み - 2018
外部発表はい

!!!All Science Journal Classification (ASJC) codes

  • 獣医学(全般)

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