Identification of the female-determining region of the W chromosome in Bombyx mori

H. Abe, T. Fujii, N. Tanaka, T. Yokoyama, H. Kakehashi, M. Ajimura, K. Mita, Yutaka Banno, Y. Yasukochi, T. Oshiki, M. Nenoi, T. Ishikawa, T. Shimada

研究成果: ジャーナルへの寄稿記事

30 引用 (Scopus)

抄録

The W chromosome of the silkworm Bombyx mori is devoid of functional genes, except for the putative female-determining gene (Fem). To localize Fem, we investigated the presence of W-specific DNA markers on strains in which an autosomal fragment containing dominant marker genes was attached to the W chromosome. We produced new W-chromosomal fragments from the existing Zebra-W strain (T(W;3)Ze chromosome) by X-irradiation, and then carried out deletion mapping of these and sex-limited yellow cocoon strains (T(W;2)Y-Chu, -Abe and -Ban types) from different Japanese stock centers. Of 12 RAPD markers identified in the normal W chromosomes of most silkworm strains in Japan, the newly irradiated W(B-YL-YS)Ze chromosome contained three, the T(W;2)Y-Chu chromosome contained six, and the T(W;2)Y-Abe and -Ban chromosomes contained only one (W-Rikishi). To investigate the ability of the reduced W-chromosome translocation fragments to form heterochromatin bodies, which are found in nuclei of normal adult female sucking stomachs, we examined cells of the normal type p50 strain and the T(W;2)Y-Chu and -Abe strains. A single sex heterochromatin body was found in nuclei of p50 females, whereas we detected only small sex heterochromatin bodies in the T(W;2)Y-Chu strain and no sex heterochromatin body in the T(W;2)Y-Abe strain. Since adult females of all strains were normal and fertile, we conclude that only extremely limited region, containing the W-Rikishi RAPD sequence of the W chromosome, is required to determine femaleness. Based on a comparison of the normal W-chromosome and 7 translocation and W-deletion strains we present a map of Fem relative to the 12 W-specific RAPD markers.

元の言語英語
ページ(範囲)269-282
ページ数14
ジャーナルGenetica
133
発行部数3
DOI
出版物ステータス出版済み - 7 1 2008

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W chromosome
Bombyx
Bombyx mori
Chromosomes
Heterochromatin
heterochromatin
gender
silkworms
chromosomes
Dominant Genes
Chromosomes, Human, Pair 3
Chromosomes, Human, Pair 7
Equidae
Y Chromosome
X Chromosome
Genetic Markers
Genes
zebras
genetic markers
Stomach

All Science Journal Classification (ASJC) codes

  • Animal Science and Zoology
  • Genetics
  • Plant Science
  • Insect Science

これを引用

Abe, H., Fujii, T., Tanaka, N., Yokoyama, T., Kakehashi, H., Ajimura, M., ... Shimada, T. (2008). Identification of the female-determining region of the W chromosome in Bombyx mori. Genetica, 133(3), 269-282. https://doi.org/10.1007/s10709-007-9210-1

Identification of the female-determining region of the W chromosome in Bombyx mori. / Abe, H.; Fujii, T.; Tanaka, N.; Yokoyama, T.; Kakehashi, H.; Ajimura, M.; Mita, K.; Banno, Yutaka; Yasukochi, Y.; Oshiki, T.; Nenoi, M.; Ishikawa, T.; Shimada, T.

:: Genetica, 巻 133, 番号 3, 01.07.2008, p. 269-282.

研究成果: ジャーナルへの寄稿記事

Abe, H, Fujii, T, Tanaka, N, Yokoyama, T, Kakehashi, H, Ajimura, M, Mita, K, Banno, Y, Yasukochi, Y, Oshiki, T, Nenoi, M, Ishikawa, T & Shimada, T 2008, 'Identification of the female-determining region of the W chromosome in Bombyx mori', Genetica, 巻. 133, 番号 3, pp. 269-282. https://doi.org/10.1007/s10709-007-9210-1
Abe H, Fujii T, Tanaka N, Yokoyama T, Kakehashi H, Ajimura M その他. Identification of the female-determining region of the W chromosome in Bombyx mori. Genetica. 2008 7 1;133(3):269-282. https://doi.org/10.1007/s10709-007-9210-1
Abe, H. ; Fujii, T. ; Tanaka, N. ; Yokoyama, T. ; Kakehashi, H. ; Ajimura, M. ; Mita, K. ; Banno, Yutaka ; Yasukochi, Y. ; Oshiki, T. ; Nenoi, M. ; Ishikawa, T. ; Shimada, T. / Identification of the female-determining region of the W chromosome in Bombyx mori. :: Genetica. 2008 ; 巻 133, 番号 3. pp. 269-282.
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abstract = "The W chromosome of the silkworm Bombyx mori is devoid of functional genes, except for the putative female-determining gene (Fem). To localize Fem, we investigated the presence of W-specific DNA markers on strains in which an autosomal fragment containing dominant marker genes was attached to the W chromosome. We produced new W-chromosomal fragments from the existing Zebra-W strain (T(W;3)Ze chromosome) by X-irradiation, and then carried out deletion mapping of these and sex-limited yellow cocoon strains (T(W;2)Y-Chu, -Abe and -Ban types) from different Japanese stock centers. Of 12 RAPD markers identified in the normal W chromosomes of most silkworm strains in Japan, the newly irradiated W(B-YL-YS)Ze chromosome contained three, the T(W;2)Y-Chu chromosome contained six, and the T(W;2)Y-Abe and -Ban chromosomes contained only one (W-Rikishi). To investigate the ability of the reduced W-chromosome translocation fragments to form heterochromatin bodies, which are found in nuclei of normal adult female sucking stomachs, we examined cells of the normal type p50 strain and the T(W;2)Y-Chu and -Abe strains. A single sex heterochromatin body was found in nuclei of p50 females, whereas we detected only small sex heterochromatin bodies in the T(W;2)Y-Chu strain and no sex heterochromatin body in the T(W;2)Y-Abe strain. Since adult females of all strains were normal and fertile, we conclude that only extremely limited region, containing the W-Rikishi RAPD sequence of the W chromosome, is required to determine femaleness. Based on a comparison of the normal W-chromosome and 7 translocation and W-deletion strains we present a map of Fem relative to the 12 W-specific RAPD markers.",
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AU - Abe, H.

AU - Fujii, T.

AU - Tanaka, N.

AU - Yokoyama, T.

AU - Kakehashi, H.

AU - Ajimura, M.

AU - Mita, K.

AU - Banno, Yutaka

AU - Yasukochi, Y.

AU - Oshiki, T.

AU - Nenoi, M.

AU - Ishikawa, T.

AU - Shimada, T.

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N2 - The W chromosome of the silkworm Bombyx mori is devoid of functional genes, except for the putative female-determining gene (Fem). To localize Fem, we investigated the presence of W-specific DNA markers on strains in which an autosomal fragment containing dominant marker genes was attached to the W chromosome. We produced new W-chromosomal fragments from the existing Zebra-W strain (T(W;3)Ze chromosome) by X-irradiation, and then carried out deletion mapping of these and sex-limited yellow cocoon strains (T(W;2)Y-Chu, -Abe and -Ban types) from different Japanese stock centers. Of 12 RAPD markers identified in the normal W chromosomes of most silkworm strains in Japan, the newly irradiated W(B-YL-YS)Ze chromosome contained three, the T(W;2)Y-Chu chromosome contained six, and the T(W;2)Y-Abe and -Ban chromosomes contained only one (W-Rikishi). To investigate the ability of the reduced W-chromosome translocation fragments to form heterochromatin bodies, which are found in nuclei of normal adult female sucking stomachs, we examined cells of the normal type p50 strain and the T(W;2)Y-Chu and -Abe strains. A single sex heterochromatin body was found in nuclei of p50 females, whereas we detected only small sex heterochromatin bodies in the T(W;2)Y-Chu strain and no sex heterochromatin body in the T(W;2)Y-Abe strain. Since adult females of all strains were normal and fertile, we conclude that only extremely limited region, containing the W-Rikishi RAPD sequence of the W chromosome, is required to determine femaleness. Based on a comparison of the normal W-chromosome and 7 translocation and W-deletion strains we present a map of Fem relative to the 12 W-specific RAPD markers.

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