Identification of the goldfish 20S proteasome β6 subunit bound to nuclear matrix

Mika Tokumoto, Akihiko Yamaguchi, Yoshitaka Nagahama, Toshinobu Tokumoto

研究成果: ジャーナルへの寄稿学術誌査読

4 被引用数 (Scopus)

抄録

Proteasomes are large, multisubunit particles that act as the proteolytic machinery for most of the regulated intracellular protein breakdown in eukaryotic cells. Proteasomes are present in both the nucleus and cytoplasm. When we analyzed the molecular composition of protein constituents of the nuclear matrix preparation of goldfish oocytes by two-dimensional polyacrylamide gel electrophoresis followed by sequence analysis, we found a 26 kDa spot identical in amino acid sequence to the β6 subunits of the 20S proteasome. No spot of other subunits of 20S proteasome was detected. Here we describe the cloning, sequencing and expression analysis of Carassius auratus, β6.

本文言語英語
ページ(範囲)62-66
ページ数5
ジャーナルFEBS Letters
472
1
DOI
出版ステータス出版済み - 4月 21 2000
外部発表はい

!!!All Science Journal Classification (ASJC) codes

  • 生物理学
  • 構造生物学
  • 生化学
  • 分子生物学
  • 遺伝学
  • 細胞生物学

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