Identification of the Novel Tooth-Specific Transcription Factor AmeloD

B. He, Y. Chiba, H. Li, S. de Vega, K. Tanaka, K. Yoshizaki, M. Ishijima, K. Yuasa, M. Ishikawa, C. Rhodes, K. Sakai, P. Zhang, S. Fukumoto, X. Zhou, Y. Yamada

研究成果: ジャーナルへの寄稿記事

1 引用 (Scopus)

抄録

Basic-helix-loop-helix (bHLH) transcription factors play an important role in various organs’ development; however, a tooth-specific bHLH factor has not been reported. In this study, we identified a novel tooth-specific bHLH transcription factor, which we named AmeloD, by screening a tooth germ complementary DNA (cDNA) library using a yeast 2-hybrid system. AmeloD was mapped onto the mouse chromosome 1q32. Phylogenetic analysis showed that AmeloD belongs to the achaete-scute complex-like (ASCL) gene family and is a homologue of ASCL5. AmeloD was uniquely expressed in the inner enamel epithelium (IEE), but its expression was suppressed after IEE cell differentiation into ameloblasts. Furthermore, AmeloD expression showed an inverse expression pattern with the epithelial cell-specific cell–cell adhesion molecule E-cadherin in the dental epithelium. Overexpression of AmeloD in dental epithelial cell line CLDE cells resulted in E-cadherin suppression. We found that AmeloD bound to E-box cis-regulatory elements in the proximal promoter region of the E-cadherin gene. These results reveal that AmeloD functions as a suppressor of E-cadherin transcription in IEE cells. Our study demonstrated that AmeloD is a novel tooth-specific bHLH transcription factor that may regulate tooth development through the suppression of E-cadherin in IEE cells.

元の言語英語
ページ(範囲)234-241
ページ数8
ジャーナルJournal of Dental Research
98
発行部数2
DOI
出版物ステータス出版済み - 2 1 2019

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Cadherins
Tooth
Transcription Factors
Dental Enamel
Basic Helix-Loop-Helix Transcription Factors
Epithelium
Achaete-Scute Complex Genome Region
Epithelial Cells
Tooth Germ
Ameloblasts
Gene Library
Genetic Promoter Regions
Cell Differentiation
Complementary DNA
Chromosomes
Yeasts
Cell Line
Genes

All Science Journal Classification (ASJC) codes

  • Dentistry(all)

これを引用

Identification of the Novel Tooth-Specific Transcription Factor AmeloD. / He, B.; Chiba, Y.; Li, H.; de Vega, S.; Tanaka, K.; Yoshizaki, K.; Ishijima, M.; Yuasa, K.; Ishikawa, M.; Rhodes, C.; Sakai, K.; Zhang, P.; Fukumoto, S.; Zhou, X.; Yamada, Y.

:: Journal of Dental Research, 巻 98, 番号 2, 01.02.2019, p. 234-241.

研究成果: ジャーナルへの寄稿記事

He, B, Chiba, Y, Li, H, de Vega, S, Tanaka, K, Yoshizaki, K, Ishijima, M, Yuasa, K, Ishikawa, M, Rhodes, C, Sakai, K, Zhang, P, Fukumoto, S, Zhou, X & Yamada, Y 2019, 'Identification of the Novel Tooth-Specific Transcription Factor AmeloD', Journal of Dental Research, 巻. 98, 番号 2, pp. 234-241. https://doi.org/10.1177/0022034518808254
He, B. ; Chiba, Y. ; Li, H. ; de Vega, S. ; Tanaka, K. ; Yoshizaki, K. ; Ishijima, M. ; Yuasa, K. ; Ishikawa, M. ; Rhodes, C. ; Sakai, K. ; Zhang, P. ; Fukumoto, S. ; Zhou, X. ; Yamada, Y. / Identification of the Novel Tooth-Specific Transcription Factor AmeloD. :: Journal of Dental Research. 2019 ; 巻 98, 番号 2. pp. 234-241.
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