Identification of thermo tolerant lactic acid bacteria isolated from silage prepared in the hot and humid climate of Southwestern Japan

Katsumi Doi, Yousuke Nishizaki, Hidetoshi Kimura, Maki Kitahara, Yasuhiro Fujino, Sadahiro Ohmomo, Toshihisa Ohshima, Seiya Ogata

研究成果: ジャーナルへの寄稿記事

8 引用 (Scopus)

抄録

To develop high-quality silage starters adapted to hot and humid weather, 12 LAB isolates from silage produced in Kyushu and Okinawa, Japan were characterized based on their morphological features, growth curves and sugar utilization. In addition, the nucleotide sequences of the V2-V3 region of their 16S rRNA genes and the 16S-23S rRNA intergenic spacer (ITS) regions were determined. The isolates were also subjected to RAPD-PCR analysis, DNA-DNA hybridization, G+C content analysis and immuno-identification using species-specific monoclonal antibodies and SDS-PAGE profiling. Nearly all of the isolates exhibited high thermotolerance and rapid growth. Combining ITS sequence analysis, RAPD-PCR and immuno-identification enabled rapid and accurate identification of closely related LAB strains that other methods failed to appropriately differentiate; for example, L. plantarum was distinguished from L. pentosus, and L. casei was distinguished from L. rhamonsus. Using the aforementioned techniques, the isolated strains were identified as L. plantarum, L. rhamonsus, L. rapi, Pediococcus pentosaceus and P. lolii. Our findings also showed that there is greater diversity among thermophilic LABs in silage prepared in a hot and humid environment.

元の言語英語
ページ(範囲)1-12
ページ数12
ジャーナルSpringerPlus
2
発行部数1
DOI
出版物ステータス出版済み - 11 14 2013

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Silage
Climate
Random Amplified Polymorphic DNA Technique
Lactic Acid
Japan
Intergenic DNA
Bacteria
DNA
Weather
Base Composition
Growth
rRNA Genes
Sequence Analysis
Polyacrylamide Gel Electrophoresis
Monoclonal Antibodies

All Science Journal Classification (ASJC) codes

  • General

これを引用

Identification of thermo tolerant lactic acid bacteria isolated from silage prepared in the hot and humid climate of Southwestern Japan. / Doi, Katsumi; Nishizaki, Yousuke; Kimura, Hidetoshi; Kitahara, Maki; Fujino, Yasuhiro; Ohmomo, Sadahiro; Ohshima, Toshihisa; Ogata, Seiya.

:: SpringerPlus, 巻 2, 番号 1, 14.11.2013, p. 1-12.

研究成果: ジャーナルへの寄稿記事

Doi, Katsumi ; Nishizaki, Yousuke ; Kimura, Hidetoshi ; Kitahara, Maki ; Fujino, Yasuhiro ; Ohmomo, Sadahiro ; Ohshima, Toshihisa ; Ogata, Seiya. / Identification of thermo tolerant lactic acid bacteria isolated from silage prepared in the hot and humid climate of Southwestern Japan. :: SpringerPlus. 2013 ; 巻 2, 番号 1. pp. 1-12.
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