Identification of whey acidic protein (WAP) in Dog Milk

Mami Seki, Rina Matsura, Tokuko Iwamori, Naoko Nukumi, Keitaro Yamanouchi, Kiyoshi Kano, Kunihiko Naito, Hideaki Tojo

研究成果: ジャーナルへの寄稿コメント/討論査読

7 被引用数 (Scopus)


Whey acidic protein (WAP) has been identified as a major whey protein in milk of a wide range of species and reportedly plays important roles in regulating the proliferation of mammary epithelial cells. However, in some species including humans, WAP is not synthesized in the mammary gland. The presence of WAP in carnivore species has not been reported. We searched the National Center for Biotechnology Information (NCBI) database for the dog WAP gene and tried biochemically to identify WAP in dog milk. The nucleotide sequence of the examined dog genomic DNA was completely identical to that in the NCBI database and showed that the dog WAP gene, like other known functional WAP genes, has four exons. Biochemical analysis of milk protein by reverse-phase HPLC and Western blotting demonstrated the presence of WAP in dog milk.

ジャーナルExperimental Animals
出版ステータス出版済み - 2012

!!!All Science Journal Classification (ASJC) codes

  • 動物科学および動物学
  • 生化学、遺伝学、分子生物学(全般)
  • 獣医学(全般)


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