IL-1α stimulates cathepsin K expression in osteoclasts via the tyrosine kinase-NF-κB pathway

S. Kamolmatyakul, W. Chen, S. Yang, Y. Abe, Ryoji Moroi, A. M. Ashique, Y. P. Li

研究成果: ジャーナルへの寄稿記事

21 引用 (Scopus)

抄録

Interleukin-1α (IL-1α) is a powerful activator of osteoclast cells. However, the underlying mechanism for this activation is unknown. In this study, we reveal that IL-1α up-regulates the expression of cathepsin K protein, a key protease in bone resorption, by five-fold. Northern blot analysis and promoter analysis show that this induction occurs at the transcriptional level, in a dose-responsive and time-dependent manner. No increase in expression occurs in the presence of either pyrrolidine dithiocarbamate (PDTC), a selective inhibitor of NF-κB, or Genistein, a protein tyrosine kinase inhibitor, suggesting that IL-1α up-regulation may be via the tyrosine kinase-NF-κB pathway to regulate cathepsin K expression. Antisense oligonucleotides to p65, but not the p50 subunit of NF-κB, suppress the IL-1α-induced expression of cathepsin K. We therefore conclude that IL-1α up-regulates cathepsin K gene expression at the transcription level, and this regulation may be via the tyrosine-kinase-NF-κB pathway.

元の言語英語
ページ(範囲)791-796
ページ数6
ジャーナルJournal of Dental Research
83
発行部数10
DOI
出版物ステータス出版済み - 1 1 2004
外部発表Yes

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Cathepsin K
Osteoclasts
Interleukin-1
Protein-Tyrosine Kinases
Up-Regulation
Genistein
Antisense Oligonucleotides
Protein Kinase Inhibitors
Bone Resorption
Northern Blotting
Peptide Hydrolases
Gene Expression
Proteins

All Science Journal Classification (ASJC) codes

  • Dentistry(all)

これを引用

IL-1α stimulates cathepsin K expression in osteoclasts via the tyrosine kinase-NF-κB pathway. / Kamolmatyakul, S.; Chen, W.; Yang, S.; Abe, Y.; Moroi, Ryoji; Ashique, A. M.; Li, Y. P.

:: Journal of Dental Research, 巻 83, 番号 10, 01.01.2004, p. 791-796.

研究成果: ジャーナルへの寄稿記事

Kamolmatyakul, S. ; Chen, W. ; Yang, S. ; Abe, Y. ; Moroi, Ryoji ; Ashique, A. M. ; Li, Y. P. / IL-1α stimulates cathepsin K expression in osteoclasts via the tyrosine kinase-NF-κB pathway. :: Journal of Dental Research. 2004 ; 巻 83, 番号 10. pp. 791-796.
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AU - Yang, S.

AU - Abe, Y.

AU - Moroi, Ryoji

AU - Ashique, A. M.

AU - Li, Y. P.

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AB - Interleukin-1α (IL-1α) is a powerful activator of osteoclast cells. However, the underlying mechanism for this activation is unknown. In this study, we reveal that IL-1α up-regulates the expression of cathepsin K protein, a key protease in bone resorption, by five-fold. Northern blot analysis and promoter analysis show that this induction occurs at the transcriptional level, in a dose-responsive and time-dependent manner. No increase in expression occurs in the presence of either pyrrolidine dithiocarbamate (PDTC), a selective inhibitor of NF-κB, or Genistein, a protein tyrosine kinase inhibitor, suggesting that IL-1α up-regulation may be via the tyrosine kinase-NF-κB pathway to regulate cathepsin K expression. Antisense oligonucleotides to p65, but not the p50 subunit of NF-κB, suppress the IL-1α-induced expression of cathepsin K. We therefore conclude that IL-1α up-regulates cathepsin K gene expression at the transcription level, and this regulation may be via the tyrosine-kinase-NF-κB pathway.

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