Imaging mass spectroscopy delineates the thinned and thickened walls of intracranial aneurysms

Taichi Ikedo, Manabu Minami, Hiroharu Kataoka, Kosuke Hayashi, Manabu Nagata, Risako Fujikawa, Fumiyoshi Yamazaki, Mitsutoshi Setou, Masayuki Yokode, Susumu Miyamoto

研究成果: ジャーナルへの寄稿記事

1 引用 (Scopus)

抄録

Object The wall thickness of intracranial aneurysms (IAs) is heterogeneous. Although thinning of the IA wall is thought to contribute to IA rupture, the underlying mechanism remains poorly understood. Recently, imaging mass spectroscopy (IMS) has been used to reveal the distribution of phospholipids in vascular diseases. To investigate the feature of phospholipid composition of IA walls, we conducted IMS in a rat model of experimentally induced IA. Material and methods IAs were surgically induced in 7-week-old male rats and analyzed by IMS in negative-ion mode. Results A molecule at m/z 885.5 was more abundant in the thickened wall than in the thinned wall (P = 0.03). Multiple-stage mass spectroscopy revealed the molecule to be phosphatidylinositol containing stearic acid and arachidonic acid (PI 18:0/20:4). Immunohistochemistry indicated that vascular smooth muscle cells (SMCs) in the thickened wall had dedifferentiated phenotypes. To investigate the relationship between accumulation of PI (18:0/20:4) and phenotypic changes in SMCs, we subjected primary mouse aortic SMCs to liquid chromatography–tandem mass spectrometry. Notably, dedifferentiated SMCs had 1.3-fold more PI (18:0/20:4) than partly differentiated SMCs. Conclusions Our study demonstrated the heterogeneity in phospholipid composition of the aneurysmal walls using experimentally induced IAs. PI (18:0/20:4) accumulated at high levels in the thickened aneurysmal wall where synthetic dedifferentiated SMCs exist, suggesting that this phospholipid may be involved in the phenotypic switching of medial SMCs in the IA wall.

元の言語英語
ページ(範囲)332-338
ページ数7
ジャーナルBiochemical and Biophysical Research Communications
495
発行部数1
DOI
出版物ステータス出版済み - 1 1 2018

Fingerprint

Intracranial Aneurysm
Muscle
Mass Spectrometry
Smooth Muscle Myocytes
Spectroscopy
Imaging techniques
Phospholipids
Rats
Molecules
Phosphatidylinositols
Chemical analysis
Arachidonic Acid
Mass spectrometry
Vascular Diseases
Vascular Smooth Muscle
Negative ions
Rupture
Cells
Immunohistochemistry
Ions

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

これを引用

Imaging mass spectroscopy delineates the thinned and thickened walls of intracranial aneurysms. / Ikedo, Taichi; Minami, Manabu; Kataoka, Hiroharu; Hayashi, Kosuke; Nagata, Manabu; Fujikawa, Risako; Yamazaki, Fumiyoshi; Setou, Mitsutoshi; Yokode, Masayuki; Miyamoto, Susumu.

:: Biochemical and Biophysical Research Communications, 巻 495, 番号 1, 01.01.2018, p. 332-338.

研究成果: ジャーナルへの寄稿記事

Ikedo, T, Minami, M, Kataoka, H, Hayashi, K, Nagata, M, Fujikawa, R, Yamazaki, F, Setou, M, Yokode, M & Miyamoto, S 2018, 'Imaging mass spectroscopy delineates the thinned and thickened walls of intracranial aneurysms', Biochemical and Biophysical Research Communications, 巻. 495, 番号 1, pp. 332-338. https://doi.org/10.1016/j.bbrc.2017.10.133
Ikedo, Taichi ; Minami, Manabu ; Kataoka, Hiroharu ; Hayashi, Kosuke ; Nagata, Manabu ; Fujikawa, Risako ; Yamazaki, Fumiyoshi ; Setou, Mitsutoshi ; Yokode, Masayuki ; Miyamoto, Susumu. / Imaging mass spectroscopy delineates the thinned and thickened walls of intracranial aneurysms. :: Biochemical and Biophysical Research Communications. 2018 ; 巻 495, 番号 1. pp. 332-338.
@article{cb77dba6288b4e449628501a998e23d4,
title = "Imaging mass spectroscopy delineates the thinned and thickened walls of intracranial aneurysms",
abstract = "Object The wall thickness of intracranial aneurysms (IAs) is heterogeneous. Although thinning of the IA wall is thought to contribute to IA rupture, the underlying mechanism remains poorly understood. Recently, imaging mass spectroscopy (IMS) has been used to reveal the distribution of phospholipids in vascular diseases. To investigate the feature of phospholipid composition of IA walls, we conducted IMS in a rat model of experimentally induced IA. Material and methods IAs were surgically induced in 7-week-old male rats and analyzed by IMS in negative-ion mode. Results A molecule at m/z 885.5 was more abundant in the thickened wall than in the thinned wall (P = 0.03). Multiple-stage mass spectroscopy revealed the molecule to be phosphatidylinositol containing stearic acid and arachidonic acid (PI 18:0/20:4). Immunohistochemistry indicated that vascular smooth muscle cells (SMCs) in the thickened wall had dedifferentiated phenotypes. To investigate the relationship between accumulation of PI (18:0/20:4) and phenotypic changes in SMCs, we subjected primary mouse aortic SMCs to liquid chromatography–tandem mass spectrometry. Notably, dedifferentiated SMCs had 1.3-fold more PI (18:0/20:4) than partly differentiated SMCs. Conclusions Our study demonstrated the heterogeneity in phospholipid composition of the aneurysmal walls using experimentally induced IAs. PI (18:0/20:4) accumulated at high levels in the thickened aneurysmal wall where synthetic dedifferentiated SMCs exist, suggesting that this phospholipid may be involved in the phenotypic switching of medial SMCs in the IA wall.",
author = "Taichi Ikedo and Manabu Minami and Hiroharu Kataoka and Kosuke Hayashi and Manabu Nagata and Risako Fujikawa and Fumiyoshi Yamazaki and Mitsutoshi Setou and Masayuki Yokode and Susumu Miyamoto",
year = "2018",
month = "1",
day = "1",
doi = "10.1016/j.bbrc.2017.10.133",
language = "English",
volume = "495",
pages = "332--338",
journal = "Biochemical and Biophysical Research Communications",
issn = "0006-291X",
publisher = "Academic Press Inc.",
number = "1",

}

TY - JOUR

T1 - Imaging mass spectroscopy delineates the thinned and thickened walls of intracranial aneurysms

AU - Ikedo, Taichi

AU - Minami, Manabu

AU - Kataoka, Hiroharu

AU - Hayashi, Kosuke

AU - Nagata, Manabu

AU - Fujikawa, Risako

AU - Yamazaki, Fumiyoshi

AU - Setou, Mitsutoshi

AU - Yokode, Masayuki

AU - Miyamoto, Susumu

PY - 2018/1/1

Y1 - 2018/1/1

N2 - Object The wall thickness of intracranial aneurysms (IAs) is heterogeneous. Although thinning of the IA wall is thought to contribute to IA rupture, the underlying mechanism remains poorly understood. Recently, imaging mass spectroscopy (IMS) has been used to reveal the distribution of phospholipids in vascular diseases. To investigate the feature of phospholipid composition of IA walls, we conducted IMS in a rat model of experimentally induced IA. Material and methods IAs were surgically induced in 7-week-old male rats and analyzed by IMS in negative-ion mode. Results A molecule at m/z 885.5 was more abundant in the thickened wall than in the thinned wall (P = 0.03). Multiple-stage mass spectroscopy revealed the molecule to be phosphatidylinositol containing stearic acid and arachidonic acid (PI 18:0/20:4). Immunohistochemistry indicated that vascular smooth muscle cells (SMCs) in the thickened wall had dedifferentiated phenotypes. To investigate the relationship between accumulation of PI (18:0/20:4) and phenotypic changes in SMCs, we subjected primary mouse aortic SMCs to liquid chromatography–tandem mass spectrometry. Notably, dedifferentiated SMCs had 1.3-fold more PI (18:0/20:4) than partly differentiated SMCs. Conclusions Our study demonstrated the heterogeneity in phospholipid composition of the aneurysmal walls using experimentally induced IAs. PI (18:0/20:4) accumulated at high levels in the thickened aneurysmal wall where synthetic dedifferentiated SMCs exist, suggesting that this phospholipid may be involved in the phenotypic switching of medial SMCs in the IA wall.

AB - Object The wall thickness of intracranial aneurysms (IAs) is heterogeneous. Although thinning of the IA wall is thought to contribute to IA rupture, the underlying mechanism remains poorly understood. Recently, imaging mass spectroscopy (IMS) has been used to reveal the distribution of phospholipids in vascular diseases. To investigate the feature of phospholipid composition of IA walls, we conducted IMS in a rat model of experimentally induced IA. Material and methods IAs were surgically induced in 7-week-old male rats and analyzed by IMS in negative-ion mode. Results A molecule at m/z 885.5 was more abundant in the thickened wall than in the thinned wall (P = 0.03). Multiple-stage mass spectroscopy revealed the molecule to be phosphatidylinositol containing stearic acid and arachidonic acid (PI 18:0/20:4). Immunohistochemistry indicated that vascular smooth muscle cells (SMCs) in the thickened wall had dedifferentiated phenotypes. To investigate the relationship between accumulation of PI (18:0/20:4) and phenotypic changes in SMCs, we subjected primary mouse aortic SMCs to liquid chromatography–tandem mass spectrometry. Notably, dedifferentiated SMCs had 1.3-fold more PI (18:0/20:4) than partly differentiated SMCs. Conclusions Our study demonstrated the heterogeneity in phospholipid composition of the aneurysmal walls using experimentally induced IAs. PI (18:0/20:4) accumulated at high levels in the thickened aneurysmal wall where synthetic dedifferentiated SMCs exist, suggesting that this phospholipid may be involved in the phenotypic switching of medial SMCs in the IA wall.

UR - http://www.scopus.com/inward/record.url?scp=85033365204&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85033365204&partnerID=8YFLogxK

U2 - 10.1016/j.bbrc.2017.10.133

DO - 10.1016/j.bbrc.2017.10.133

M3 - Article

C2 - 29111330

AN - SCOPUS:85033365204

VL - 495

SP - 332

EP - 338

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

SN - 0006-291X

IS - 1

ER -