Immobilization of bacterial luciferase into poly(n-isopropylacrylamide) film for electrochemical control of a bioluminescence reaction

Yoji Kawanami, Shinya Yamasaki, Shuto Yamada, Kô Takehara

研究成果: Contribution to journalArticle査読

4 被引用数 (Scopus)

抄録

A poly(N-isopropylacrylamide) film was modified on an indium-tin oxide electrode in order to immobilize bacterial luciferase (BL) on the electrode surface. By using the modified electrode, flavin mononucleotide (FMN) was electrochemically reduced to FMNH2, which is one of the substrates of the BL luminescence reaction, to control the bioluminescence reaction by BL. The BL reaction in the modified film could be promoted and controlled by the electrochemical generation of FMNH 2 . This BL luminescence system was evaluated as a model system for the inhibitory assay of hydrophobic molecules on protein functions. 2012

本文言語英語
ページ(範囲)1013-1015
ページ数3
ジャーナルanalytical sciences
28
10
DOI
出版ステータス出版済み - 2012

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry

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