Immobilization of surfactant-lipase complexes and their high heat resistance in organic media

Muneharu Goto, Chiaki Hatanaka, Masahiro Goto

    研究成果: ジャーナルへの寄稿学術誌査読

    23 被引用数 (Scopus)

    抄録

    Surfactant-lipase complexes were immobilized in an n-vinyl-2-pyrrolidone gel matrix. Features of a native lipase and gel-immobilized surfactant-lipase complexes were measured by esterification reaction between lauric acid and benzyl alcohol in isooctane. Optimal gel-immobilized surfactant-lipase complex activity was 37.2 mol h-1 kg-1-lipase. Gel-immobilized lipase complexes showed a 51-fold increase in activity and exhibited superior heat resistance compared to native lipases. The optimum temperature for the immobilized lipase complex was 60°C, as compared to 37°C for native lipase activity. Gel-immobilized lipase complexes could be readily recovered, and their high activity was completely preserved, even after 10 reuses.

    本文言語英語
    ページ(範囲)91-94
    ページ数4
    ジャーナルBiochemical Engineering Journal
    24
    1
    DOI
    出版ステータス出版済み - 5月 15 2005

    !!!All Science Journal Classification (ASJC) codes

    • バイオテクノロジー
    • 環境工学
    • バイオエンジニアリング
    • 生体医工学

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