Immune therapeutic potential of stem cells from human supernumerary teeth

Y. Makino, Haruyoshi Yamaza, K. Akiyama, L. Ma, Y. Hoshino, Kazuaki Nonaka, Y. Terada, Toshio Kukita, S. Shi, Takayoshi Yamaza

研究成果: ジャーナルへの寄稿記事

22 引用 (Scopus)

抄録

Discoveries of immunomodulatory functions in mesenchymal stem cells (MSCs) have suggested that they might have therapeutic utility in treating immune diseases. Recently, a novel MSC population was identified from dental pulp of human supernumerary teeth, and its multipotency characterized. Herein, we first examined the in vitro and in vivo immunomodulatory functions of human supernumerary tooth-derived stem cells (SNTSCs). SNTSCs suppressed not only the viability of T-cells, but also the differentiation of interleukin 17 (IL-17)-secreting helper T (Th17) -cells in in vitro co-culture experiments. In addition, systemic SNTSC transplantation ameliorated the shortened lifespan and elevated serum autoantibodies and nephritis-like renal dysfunction in systemic lupus erythematosus (SLE) model MRL/lpr mice. SNTSC transplantation also suppressed in vivo increased levels of peripheral Th17 cells and IL-17, as well as ex vivo differentiation of Th17 cells in MRL/lpr mice. Adoptive transfer experiments demonstrated that SNTSC-transplanted MRL/lpr mouse-derived T-cell-adopted immunocompromised mice showed a longer lifespan in comparison with non-transplanted MRL/lpr mouse-derived T-cell-adopted immunocompromised mice, indicating that SNTSC transplantation suppresses the hyper-immune condition of MRL/lpr mice through suppressing T-cells. Analysis of these data suggests that SNTSCs are a promising MSC source for cell-based therapy for immune diseases such as SLE.

元の言語英語
ページ(範囲)609-615
ページ数7
ジャーナルJournal of Dental Research
92
発行部数7
DOI
出版物ステータス出版済み - 7 1 2013

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Supernumerary Tooth
Inbred MRL lpr Mouse
Stem Cells
Th17 Cells
Stem Cell Transplantation
Mesenchymal Stromal Cells
T-Lymphocytes
Interleukin-17
Immune System Diseases
Therapeutics
Systemic Lupus Erythematosus
Dental Pulp
Adoptive Transfer
Nephritis
Cell- and Tissue-Based Therapy
Helper-Inducer T-Lymphocytes
Coculture Techniques
Autoantibodies
Cell Differentiation
Kidney

All Science Journal Classification (ASJC) codes

  • Dentistry(all)

これを引用

Immune therapeutic potential of stem cells from human supernumerary teeth. / Makino, Y.; Yamaza, Haruyoshi; Akiyama, K.; Ma, L.; Hoshino, Y.; Nonaka, Kazuaki; Terada, Y.; Kukita, Toshio; Shi, S.; Yamaza, Takayoshi.

:: Journal of Dental Research, 巻 92, 番号 7, 01.07.2013, p. 609-615.

研究成果: ジャーナルへの寄稿記事

Makino, Y. ; Yamaza, Haruyoshi ; Akiyama, K. ; Ma, L. ; Hoshino, Y. ; Nonaka, Kazuaki ; Terada, Y. ; Kukita, Toshio ; Shi, S. ; Yamaza, Takayoshi. / Immune therapeutic potential of stem cells from human supernumerary teeth. :: Journal of Dental Research. 2013 ; 巻 92, 番号 7. pp. 609-615.
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abstract = "Discoveries of immunomodulatory functions in mesenchymal stem cells (MSCs) have suggested that they might have therapeutic utility in treating immune diseases. Recently, a novel MSC population was identified from dental pulp of human supernumerary teeth, and its multipotency characterized. Herein, we first examined the in vitro and in vivo immunomodulatory functions of human supernumerary tooth-derived stem cells (SNTSCs). SNTSCs suppressed not only the viability of T-cells, but also the differentiation of interleukin 17 (IL-17)-secreting helper T (Th17) -cells in in vitro co-culture experiments. In addition, systemic SNTSC transplantation ameliorated the shortened lifespan and elevated serum autoantibodies and nephritis-like renal dysfunction in systemic lupus erythematosus (SLE) model MRL/lpr mice. SNTSC transplantation also suppressed in vivo increased levels of peripheral Th17 cells and IL-17, as well as ex vivo differentiation of Th17 cells in MRL/lpr mice. Adoptive transfer experiments demonstrated that SNTSC-transplanted MRL/lpr mouse-derived T-cell-adopted immunocompromised mice showed a longer lifespan in comparison with non-transplanted MRL/lpr mouse-derived T-cell-adopted immunocompromised mice, indicating that SNTSC transplantation suppresses the hyper-immune condition of MRL/lpr mice through suppressing T-cells. Analysis of these data suggests that SNTSCs are a promising MSC source for cell-based therapy for immune diseases such as SLE.",
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