Immunoaffinity column for isolation of bioactive compounds using monoclonal antibodies

Waraporn Putalun, Noriko Fukuda, Hiroyuki Tanaka, Yukihiro Shoyama

研究成果: Contribution to journalReview article査読

1 被引用数 (Scopus)

抄録

Anti-solamargine and anti-ginsenoside Rb1 monoclonal antibodies were used for preparation of an immunoaffinity column. Total solasodine glycosides were separated directly from the crude extract of Solanum khasianum fruit, by the established immunoaffinity column. This method was specific for solasodine glycosides, which was detected by thin layer chromatography and eastern blotting. An immunoaffinity column, using antiginsenoside Rb1 monoclonal antibody, has made possible a single-step separation of ginsenoside Rb1 from a crude extract of ginseng roots (Panax ginseng).

本文言語英語
ページ(範囲)2387-2398
ページ数12
ジャーナルJournal of Liquid Chromatography and Related Technologies
25
13-15
DOI
出版ステータス出版済み - 10 10 2002

All Science Journal Classification (ASJC) codes

  • 分析化学
  • 生化学
  • 薬科学
  • 臨床生化学

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