TY - JOUR
T1 - Immunochemical analysis of the antimalarial drugs artemisinin and artesunate
AU - Tanaka, Hiroyuki
AU - Paudel, Madan K.
AU - Takei, Ayako
AU - Sakoda, Junichi
AU - Juengwatanatrakul, Thaweesak
AU - Sasaki-Tabata, Kaori
AU - Putalun, Waraporn
AU - De-Eknamkul, Wanchai
AU - Matangkasombut, Oraphan
AU - Shoyama, Yukihiro
AU - Morimoto, Satoshi
N1 - Funding Information:
This work was funded in part by Grants-in-Aid from the JSPS Asian CORE Program, the Ministry of Education, Culture, Sports, Science and Technology of Japan, and the National Center for Genetic and Biotechnology (BIOTEC), Thailand. We are also grateful for technical support from the Research Support Center, Graduate School of Medical Sciences of Kyushu University.
Publisher Copyright:
© 2012 by the authors; licensee MDPI, Basel, Switzerland.
PY - 2012/12
Y1 - 2012/12
N2 - We prepared a monoclonal antibody (mAb 1C1) showing specificity for artemisinin (AM) and artesunate (AS), and we developed an indirect competitive enzyme-linked immunosorbent assay (icELISA) using this novel mAb. Moreover, we prepared a recombinant antibody derived from mAb 1C1 in order to overcome insufficient mAb production by hybridoma culture. A recombinant antigen-binding fragment (Fab) was easily constructed using antibody manipulation technologies and was produced by microorganisms in high yield. We herein review immunochemical approaches for analysis of the antimalarial drugs AM and AS that were able to yield analysis results for multiple samples in a short period of time using simple and reliable protocols.
AB - We prepared a monoclonal antibody (mAb 1C1) showing specificity for artemisinin (AM) and artesunate (AS), and we developed an indirect competitive enzyme-linked immunosorbent assay (icELISA) using this novel mAb. Moreover, we prepared a recombinant antibody derived from mAb 1C1 in order to overcome insufficient mAb production by hybridoma culture. A recombinant antigen-binding fragment (Fab) was easily constructed using antibody manipulation technologies and was produced by microorganisms in high yield. We herein review immunochemical approaches for analysis of the antimalarial drugs AM and AS that were able to yield analysis results for multiple samples in a short period of time using simple and reliable protocols.
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U2 - 10.3390/antib1030273
DO - 10.3390/antib1030273
M3 - Short survey
AN - SCOPUS:84981303992
SN - 2073-4468
VL - 1
SP - 273
EP - 283
JO - Antibodies
JF - Antibodies
IS - 3
ER -