Immunocytochemical identification of Pit-1 containing cells in the anterior pituitary of hens

Vishwajit Surchowdhury, Yukinori Yoshimura

研究成果: ジャーナルへの寄稿記事

3 引用 (Scopus)

抄録

Our goal was to identify the cells expressing Pit-1 protein in chicken anterior pituitary. The anterior pituitaries were collected from laying hens after perfusion with formalin-PBS, and fixed with Bouin's fixative followed by paraffin embedding. Sections of the anterior pituitaries were immunostained for Pit-1 in the first staining sequence followed by staining for 6 types of pituitary hormones in the second sequence. Pit-1 positive nuclei were observed in the glandular cells in both the cephalic and caudal lobes. Pit-1 immunoreaction products were colocalized in the glandular cells immunopositive for growth hormone, thyroid-stimulating hormone, follicle-stimulating hormone, luteinizing hormone, adrenocorticotropic hormone or prolactin. These results indicate that Pit-1 protein induction occurs in 6 types of glandular cells, suggesting that Pit-1 may regulate hormone synthesis in each glandular cell in the chicken pituitary.

元の言語英語
ページ(範囲)375-379
ページ数5
ジャーナルJournal of Reproduction and Development
49
発行部数5
DOI
出版物ステータス出版済み - 12 1 2003
外部発表Yes

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anterior pituitary
hens
cells
chickens
pituitary hormones
thyrotropin
follicle-stimulating hormone
corticotropin
luteinizing hormone
prolactin
formalin
alkanes
laying hens
somatotropin
cell growth
proteins
hormones
synthesis
staining

All Science Journal Classification (ASJC) codes

  • Animal Science and Zoology

これを引用

Immunocytochemical identification of Pit-1 containing cells in the anterior pituitary of hens. / Surchowdhury, Vishwajit; Yoshimura, Yukinori.

:: Journal of Reproduction and Development, 巻 49, 番号 5, 01.12.2003, p. 375-379.

研究成果: ジャーナルへの寄稿記事

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