Immunocytochemical localization of 67 KD Ca2+ binding protein (p67) in ventricular, skeletal, and smooth muscle cells

Hiroshi Iida, T. Hatae, Y. Shibata

研究成果: ジャーナルへの寄稿記事

16 引用 (Scopus)

抄録

By using immunocytochemical techniques, we examined the localization of a 67 kDa Ca2+ binding protein (p67) and calpactin I heavy chain (p36) in ventricular myocytes, skeletal myocytes, and intestinal smooth muscle cells. Immunofluorescence microscopy revealed that the p67 was expressed in all these muscle cells, whereas anti-p36 antibody stained cells in connective tissues but failed to stain these muscle cells. Immunogold electron microscopy was carried out to examine the subcellular localization of the p67 in muscle cells. The results showed that the p67 was exclusively confined to the plasma membrane of muscle cells and the presumptive transverse tubules of the striated myocytes. Immunoblot analysis with anti-p67 antibody showed that the p67 was indeed a constitutive protein of the sarcolemma isolated from rat hearts. These results indicate that the p67 is a sarcolemma-associated Ca2+ binding protein expressed in both striated myocytes and intestinal smooth muscle cells.

元の言語英語
ページ(範囲)1899-1907
ページ数9
ジャーナルJournal of Histochemistry and Cytochemistry
40
発行部数12
DOI
出版物ステータス出版済み - 1 1 1992

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Muscle Cells
Smooth Muscle Myocytes
Carrier Proteins
Skeletal Muscle
Sarcolemma
Anti-Idiotypic Antibodies
Annexin A2
Connective Tissue Cells
Skeletal Muscle Fibers
Fluorescence Microscopy
Electron Microscopy
Coloring Agents
Cell Membrane
Proteins

All Science Journal Classification (ASJC) codes

  • Anatomy
  • Histology

これを引用

Immunocytochemical localization of 67 KD Ca2+ binding protein (p67) in ventricular, skeletal, and smooth muscle cells. / Iida, Hiroshi; Hatae, T.; Shibata, Y.

:: Journal of Histochemistry and Cytochemistry, 巻 40, 番号 12, 01.01.1992, p. 1899-1907.

研究成果: ジャーナルへの寄稿記事

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abstract = "By using immunocytochemical techniques, we examined the localization of a 67 kDa Ca2+ binding protein (p67) and calpactin I heavy chain (p36) in ventricular myocytes, skeletal myocytes, and intestinal smooth muscle cells. Immunofluorescence microscopy revealed that the p67 was expressed in all these muscle cells, whereas anti-p36 antibody stained cells in connective tissues but failed to stain these muscle cells. Immunogold electron microscopy was carried out to examine the subcellular localization of the p67 in muscle cells. The results showed that the p67 was exclusively confined to the plasma membrane of muscle cells and the presumptive transverse tubules of the striated myocytes. Immunoblot analysis with anti-p67 antibody showed that the p67 was indeed a constitutive protein of the sarcolemma isolated from rat hearts. These results indicate that the p67 is a sarcolemma-associated Ca2+ binding protein expressed in both striated myocytes and intestinal smooth muscle cells.",
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