Immunocytochemical study of the surrounding envelope of autophagic vacuoles in cultured rat hepatocytes

Koji Furuno, Toyoko Ishikawa, Kenji Akasaki, Sook Lee, Yukio Nishimura, Hiroshi Tsuji, Masaru Himeno, Keitaro Kato

研究成果: ジャーナルへの寄稿記事

45 引用 (Scopus)

抄録

By the use of electron immunoperoxidase cytochemistry at the ultrastructural level, the relationship of the surrounding sac of the autophagic vacuoles to the different cytomembranes was studied. When the endoplasmic reticulum was completely stained for microsomal carboxyesterase E1, the enzyme was not found to be labeled in the developed envelopes forming autophagic vacuoles. The autophagic envelope at the formative stages was also devoid of albumin which intensely stained Golgi cisternae. However, although it was rare, the endoplasmic reticulum showed an electronlucent region like an early autophagic envelope in its cisternae which was lacking in carboxyesterase E1. In addition, deeply curving swelled cisternae where carboxyesterase E1 was found at the edges were occasionally encountered. These observations suggest that the segregating membranes arise from an endoplasmic reticulum and the structural characteristics of the endoplasmic membranes change at very early stages of formation of autophagic vacuoles. Acid phosphatase, a lysosomal marker enzyme, began to be localized on sections of the double membranes of newly created autophagic vacuoles. The enzyme spread all along the limiting membranes of the autophagic vacuoles, while, at the same time, the double membranes were converted into a single membrane. A lysosomal membrane glycoprotein (LGP107) was also localized on the surrounding envelope of autophagic vacuoles in a fashion similar to that of acid phosphatase. Lysosomal hydrolases seem to play some role in the conversion of double limiting membranes into a single limiting membrane.

元の言語英語
ページ(範囲)261-268
ページ数8
ジャーナルExperimental Cell Research
189
発行部数2
DOI
出版物ステータス出版済み - 1 1 1990

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Vacuoles
Hepatocytes
Membranes
Carboxylesterase
Endoplasmic Reticulum
Acid Phosphatase
Enzymes
Lysosome-Associated Membrane Glycoproteins
Histocytochemistry
Hydrolases
Albumins
Electrons

All Science Journal Classification (ASJC) codes

  • Cell Biology

これを引用

Furuno, K., Ishikawa, T., Akasaki, K., Lee, S., Nishimura, Y., Tsuji, H., ... Kato, K. (1990). Immunocytochemical study of the surrounding envelope of autophagic vacuoles in cultured rat hepatocytes. Experimental Cell Research, 189(2), 261-268. https://doi.org/10.1016/0014-4827(90)90245-6

Immunocytochemical study of the surrounding envelope of autophagic vacuoles in cultured rat hepatocytes. / Furuno, Koji; Ishikawa, Toyoko; Akasaki, Kenji; Lee, Sook; Nishimura, Yukio; Tsuji, Hiroshi; Himeno, Masaru; Kato, Keitaro.

:: Experimental Cell Research, 巻 189, 番号 2, 01.01.1990, p. 261-268.

研究成果: ジャーナルへの寄稿記事

Furuno, K, Ishikawa, T, Akasaki, K, Lee, S, Nishimura, Y, Tsuji, H, Himeno, M & Kato, K 1990, 'Immunocytochemical study of the surrounding envelope of autophagic vacuoles in cultured rat hepatocytes', Experimental Cell Research, 巻. 189, 番号 2, pp. 261-268. https://doi.org/10.1016/0014-4827(90)90245-6
Furuno, Koji ; Ishikawa, Toyoko ; Akasaki, Kenji ; Lee, Sook ; Nishimura, Yukio ; Tsuji, Hiroshi ; Himeno, Masaru ; Kato, Keitaro. / Immunocytochemical study of the surrounding envelope of autophagic vacuoles in cultured rat hepatocytes. :: Experimental Cell Research. 1990 ; 巻 189, 番号 2. pp. 261-268.
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