Immunogenicity of Actinobacillus actinomycetemcomitans serotype b-specific polysaccharide-protein conjugate

N. Takamatsu-Matsushita, N. Yamaguchi, M. Kawasaki, Yoshihisa Yamashita, T. Takehara, T. Koga

研究成果: ジャーナルへの寄稿学術誌査読

13 被引用数 (Scopus)


To enhance the immunogenicity of capsular-like serotype b-specific polysaccharide antigen (SPA) of Actinobacillus actinomycetemcomitans, the purified antigen was coupled with bovine serum albumin via an adipic acid hydrazide functional group. The conjugate (SPA-bovine serum albumin) or the native SPA was administered subcutaneously or intranasally to BALB/c mice. Neither subcutaneous immunization with SPA emulsified in Freund adjuvant nor intranasal immunization with SPA and cholera toxin B subunit elicited any antibody responses to the polysaccharide antigen. High serum immunoglobulin M (IgM) and IgG responses to SPA were induced by subcutaneous immunization with SPA-bovine serum albumin in Freund adjuvant. Serum and salivary IgA responses to SPA in addition to IgM and IgG responses were induced by intranasal immunization with the conjugate and cholera toxin B subunit. To investigate the functional activity of SPA-specific antibodies, the opsonic activity of sera from BALB/c mice immunized with the conjugate was assessed by chemiluminescence assay using human polymorphonuclear leukocytes. Murine antisera efficiently opsonized A. actinomycetemcomitans serotype b in the assay, suggesting that antibodies to SPA of the organism might serve as a protective role.

ジャーナルOral Microbiology and Immunology
出版ステータス出版済み - 8月 1996

!!!All Science Journal Classification (ASJC) codes

  • 免疫学
  • 微生物学(医療)
  • 歯科学(全般)


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