Immunogold localization of photosynthetic enzymes in leaves of Aristida latifolia, a unique C4 grass with a double chlorenchymatous bundle sheath

Ueno Osamu

研究成果: ジャーナルへの寄稿記事

30 引用 (Scopus)

抄録

C4 species of the genus Aristida (Poaceae) have 3 distinct types of photosynthetic cells in their leaves, the mesophyll (M) cells, the outer bundle sheath (BS) cells, and the inner BS cells, and exhibit a unique Kranz‐type leaf anatomy. The cellular localization of C3 and C4 photosynthetic enzymes was investigated in leaves of Aristida latifolia Domin by the protein A‐gold immunocytochemical technique. The outer BS cells contained centripetally located small chloroplasts, which were structurally similar to those of the M cells. The inner BS cells contained centrifugally located large chloroplasts, which lacked well‐developed grana and exhibited rudimentary grana for the most part. The leaves contained high levels of NADP‐malic enzyme (EC 1.1.1.40) activity, and the plant was classified as being of the NADP‐malic enzyme type. The immunocytochemical study revealed that labeling of ribulose 1,5‐bisphos‐phate carboxylase/oxygenase (EC 4.1.1.39) was present in the chloroplasts of the outer and inner BS cells, but was undetectable in the M cells. Labeling of phoshoen‐olpyruvate carboxylase (EC 4.1.1.31) was observed in the cytosol of M cells, but not in that of BS cells. By contrast, labeling of pyruvate, Pi dikinase (PPDK, EC 2.7.9.1) was evident not only in the chloroplasts of M cells but also in those of outer BS cells, but was absent from the inner BS cells. The density of labeling in the chloroplasts of M cells was higher than that in chloroplasts of outer BS cells. These results indicate that the two carboxylating enzymes are differentially distributed between the M cells and the two types of BS cells, whereas PPDK shows a more complex distribution pattern. The locations of these enzymes are discussed in relation to C4 photosynthesis. 1992 Physiologia Plantarum

元の言語英語
ページ(範囲)189-196
ページ数8
ジャーナルPhysiologia Plantarum
85
発行部数2
DOI
出版物ステータス出版済み - 1 1 1992

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Aristida
bundle sheath cells
Poaceae
grasses
Enzymes
enzymes
chloroplasts
leaves
Chloroplasts
cells
grana
malate dehydrogenase (oxaloacetate-decarboxylating) (NADP)
Thylakoids
ribulose
C4 photosynthesis
phosphoenolpyruvate carboxylase
C4 plants
ribulose-bisphosphate carboxylase
oxygenases
Orthophosphate Dikinase Pyruvate

All Science Journal Classification (ASJC) codes

  • Physiology
  • Genetics
  • Plant Science
  • Cell Biology

これを引用

Immunogold localization of photosynthetic enzymes in leaves of Aristida latifolia, a unique C4 grass with a double chlorenchymatous bundle sheath. / Osamu, Ueno.

:: Physiologia Plantarum, 巻 85, 番号 2, 01.01.1992, p. 189-196.

研究成果: ジャーナルへの寄稿記事

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abstract = "C4 species of the genus Aristida (Poaceae) have 3 distinct types of photosynthetic cells in their leaves, the mesophyll (M) cells, the outer bundle sheath (BS) cells, and the inner BS cells, and exhibit a unique Kranz‐type leaf anatomy. The cellular localization of C3 and C4 photosynthetic enzymes was investigated in leaves of Aristida latifolia Domin by the protein A‐gold immunocytochemical technique. The outer BS cells contained centripetally located small chloroplasts, which were structurally similar to those of the M cells. The inner BS cells contained centrifugally located large chloroplasts, which lacked well‐developed grana and exhibited rudimentary grana for the most part. The leaves contained high levels of NADP‐malic enzyme (EC 1.1.1.40) activity, and the plant was classified as being of the NADP‐malic enzyme type. The immunocytochemical study revealed that labeling of ribulose 1,5‐bisphos‐phate carboxylase/oxygenase (EC 4.1.1.39) was present in the chloroplasts of the outer and inner BS cells, but was undetectable in the M cells. Labeling of phoshoen‐olpyruvate carboxylase (EC 4.1.1.31) was observed in the cytosol of M cells, but not in that of BS cells. By contrast, labeling of pyruvate, Pi dikinase (PPDK, EC 2.7.9.1) was evident not only in the chloroplasts of M cells but also in those of outer BS cells, but was absent from the inner BS cells. The density of labeling in the chloroplasts of M cells was higher than that in chloroplasts of outer BS cells. These results indicate that the two carboxylating enzymes are differentially distributed between the M cells and the two types of BS cells, whereas PPDK shows a more complex distribution pattern. The locations of these enzymes are discussed in relation to C4 photosynthesis. 1992 Physiologia Plantarum",
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