Immunohistochemical detection of interferon-γ-producing cells in granuloma formation of sporotrichosis

Tetsuya Koga, H. Duan, Masutaka Furue

研究成果: ジャーナルへの寄稿記事

23 引用 (Scopus)

抄録

Granuloma formation is a common response in the skin infection of sporotrichosis. The involvement of interferon-γ (IFN-γ) and inducible nitric oxide synthase (iNOS) in the granuloma formation of sporotrichosis is less known. The aim of this study was to describe by immunohistochemistry the exact tissue distribution of IFN-γ-positive cells and iNOS-positive cells in the granuloma of skin lesions from patients with sporotrichosis. Formalin-fixed paraffin-embedded skin biopsy sections from five patients were stained by immunohistochemical methods. Mixture of CD4-positive T cells and CD8-positive T cells were present in and around the granulomatous lesions. IFN-γ-positive cells, like mononuclear lymphoid cells, were detected in the periphery of the granulomas in all samples. The pattern of IFN-γ staining appeared to be a combination of intracellular staining in mononuclear lymphoid cells and extracellular deposition in the surrounding tissue. The majority of the epitheloid cells and multinucleated giant cells within the granuloma were identified by immunostaining for CD68. In contrast, no expression of immunoreactive iNOS was observed in those cells in all samples. These findings indicate that granulomas of sporotrichosis are coupled with Th1 response.

元の言語英語
ページ(範囲)111-114
ページ数4
ジャーナルMedical mycology
40
発行部数2
出版物ステータス出版済み - 5 27 2002

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Sporotrichosis
Granuloma
Interferons
Nitric Oxide Synthase Type II
Skin
Giant Cell Granuloma
Lymphocytes
Staining and Labeling
T-Lymphocytes
Tissue Distribution
Giant Cells
Paraffin
Formaldehyde
Immunohistochemistry
Biopsy
Infection

All Science Journal Classification (ASJC) codes

  • Infectious Diseases

これを引用

Immunohistochemical detection of interferon-γ-producing cells in granuloma formation of sporotrichosis. / Koga, Tetsuya; Duan, H.; Furue, Masutaka.

:: Medical mycology, 巻 40, 番号 2, 27.05.2002, p. 111-114.

研究成果: ジャーナルへの寄稿記事

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abstract = "Granuloma formation is a common response in the skin infection of sporotrichosis. The involvement of interferon-γ (IFN-γ) and inducible nitric oxide synthase (iNOS) in the granuloma formation of sporotrichosis is less known. The aim of this study was to describe by immunohistochemistry the exact tissue distribution of IFN-γ-positive cells and iNOS-positive cells in the granuloma of skin lesions from patients with sporotrichosis. Formalin-fixed paraffin-embedded skin biopsy sections from five patients were stained by immunohistochemical methods. Mixture of CD4-positive T cells and CD8-positive T cells were present in and around the granulomatous lesions. IFN-γ-positive cells, like mononuclear lymphoid cells, were detected in the periphery of the granulomas in all samples. The pattern of IFN-γ staining appeared to be a combination of intracellular staining in mononuclear lymphoid cells and extracellular deposition in the surrounding tissue. The majority of the epitheloid cells and multinucleated giant cells within the granuloma were identified by immunostaining for CD68. In contrast, no expression of immunoreactive iNOS was observed in those cells in all samples. These findings indicate that granulomas of sporotrichosis are coupled with Th1 response.",
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N2 - Granuloma formation is a common response in the skin infection of sporotrichosis. The involvement of interferon-γ (IFN-γ) and inducible nitric oxide synthase (iNOS) in the granuloma formation of sporotrichosis is less known. The aim of this study was to describe by immunohistochemistry the exact tissue distribution of IFN-γ-positive cells and iNOS-positive cells in the granuloma of skin lesions from patients with sporotrichosis. Formalin-fixed paraffin-embedded skin biopsy sections from five patients were stained by immunohistochemical methods. Mixture of CD4-positive T cells and CD8-positive T cells were present in and around the granulomatous lesions. IFN-γ-positive cells, like mononuclear lymphoid cells, were detected in the periphery of the granulomas in all samples. The pattern of IFN-γ staining appeared to be a combination of intracellular staining in mononuclear lymphoid cells and extracellular deposition in the surrounding tissue. The majority of the epitheloid cells and multinucleated giant cells within the granuloma were identified by immunostaining for CD68. In contrast, no expression of immunoreactive iNOS was observed in those cells in all samples. These findings indicate that granulomas of sporotrichosis are coupled with Th1 response.

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