Immunological Analysis of a Small GTP-Binding Protein in Higher Plant Cells

Toyoaki Anai, Evalour T. Aspuria, Nobuharu Fujii, Takashi Ueda, Minami Matsui, Koji Hasegawa, Hirofumi Uchimiya

研究成果: ジャーナルへの寄稿学術誌査読

7 被引用数 (Scopus)

抄録

ara genes encoding YPT/RAB like small GTP-binding proteins, which are involved in the vesicular transport system in various eukaryotic cells, have been isolated from Arabidopsis thaliana. To determine the subcellular localization of ARA-4 protein, suspensions of cultured transgenic tobacco cells were analyzed using monoclonal antibody to Arabidopsis thaliana ARA-4 protein. ARA-4 protein was found in the microsomal membrane fraction. Similar results were obtained withArabidopsis thaliana cells. The ARA-4 protein was readily released by detergents, such as 0.5 % sodium deoxycholate and NP-40. The results of immuno-blot analysis with extracts of different organs show that the ARA-4 protein is present in most organs of Arabidopsis thaliana plants, e. g. root, stem, flower and young fruit, at the same level in all organs except for root, where the level is lower.

本文言語英語
ページ(範囲)48-52
ページ数5
ジャーナルJournal of Plant Physiology
147
1
DOI
出版ステータス出版済み - 1995
外部発表はい

!!!All Science Journal Classification (ASJC) codes

  • 生理学
  • 農業および作物学
  • 植物科学

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