抄録
Streptococcus mutans serotype g secretes at least three kinds of glucosyltransferase with different enzymological and immunological properties. One of them is a primer-independent enzyme and seems to be the source of primer for the others, both of which are primer-dependent enzymes. Recently, we purified the primer-independent enzyme, the third glucosyltransferase in this group from S. mutans strain AHT-k serotype g. In the present study, we examined the specificity of the antiserum against the primer-independent glucosyltransferase using extracellular culture-conditioned fluids of many strains of the various serotypes of S. mutans. The antiserum cross-reacted with the extracellular culture fluids from strains of serotypes d and a, in addition to serotype g, but not with those of other serotypes, indicating that the primer-independent glucosyltransferase is secreted by the S. sobrinus and S. cricetus, but not by S. mutans and S. rattus. The antiserum did not completely inhibit the activity of the enzyme, even at more than twofold antibody excess, determined by indirect precipitation with immobilized staphylococcal protein A.
| 元の言語 | 英語 |
|---|---|
| ページ（範囲） | 1223-1227 |
| ページ数 | 5 |
| ジャーナル | Journal of General Microbiology |
| 巻 | 134 |
| 発行部数 | 5 |
| 出版物ステータス | 出版済み - 1 1 1988 |
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All Science Journal Classification (ASJC) codes
- Microbiology
これを引用
Immunological properties of the primer-independent glucosyltransferase of Streptococcus mutans serotypes d and g. / Yamashita, Y.; Shigeoka, T.; Hanada, N.; Takehara, T.
：: Journal of General Microbiology, 巻 134, 番号 5, 01.01.1988, p. 1223-1227.研究成果: ジャーナルへの寄稿 › 記事
}
TY - JOUR
T1 - Immunological properties of the primer-independent glucosyltransferase of Streptococcus mutans serotypes d and g
AU - Yamashita, Y.
AU - Shigeoka, T.
AU - Hanada, N.
AU - Takehara, T.
PY - 1988/1/1
Y1 - 1988/1/1
N2 - Streptococcus mutans serotype g secretes at least three kinds of glucosyltransferase with different enzymological and immunological properties. One of them is a primer-independent enzyme and seems to be the source of primer for the others, both of which are primer-dependent enzymes. Recently, we purified the primer-independent enzyme, the third glucosyltransferase in this group from S. mutans strain AHT-k serotype g. In the present study, we examined the specificity of the antiserum against the primer-independent glucosyltransferase using extracellular culture-conditioned fluids of many strains of the various serotypes of S. mutans. The antiserum cross-reacted with the extracellular culture fluids from strains of serotypes d and a, in addition to serotype g, but not with those of other serotypes, indicating that the primer-independent glucosyltransferase is secreted by the S. sobrinus and S. cricetus, but not by S. mutans and S. rattus. The antiserum did not completely inhibit the activity of the enzyme, even at more than twofold antibody excess, determined by indirect precipitation with immobilized staphylococcal protein A.
AB - Streptococcus mutans serotype g secretes at least three kinds of glucosyltransferase with different enzymological and immunological properties. One of them is a primer-independent enzyme and seems to be the source of primer for the others, both of which are primer-dependent enzymes. Recently, we purified the primer-independent enzyme, the third glucosyltransferase in this group from S. mutans strain AHT-k serotype g. In the present study, we examined the specificity of the antiserum against the primer-independent glucosyltransferase using extracellular culture-conditioned fluids of many strains of the various serotypes of S. mutans. The antiserum cross-reacted with the extracellular culture fluids from strains of serotypes d and a, in addition to serotype g, but not with those of other serotypes, indicating that the primer-independent glucosyltransferase is secreted by the S. sobrinus and S. cricetus, but not by S. mutans and S. rattus. The antiserum did not completely inhibit the activity of the enzyme, even at more than twofold antibody excess, determined by indirect precipitation with immobilized staphylococcal protein A.
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UR - http://www.scopus.com/inward/citedby.url?scp=0023875718&partnerID=8YFLogxK
M3 - Article
C2 - 2974068
AN - SCOPUS:0023875718
VL - 134
SP - 1223
EP - 1227
JO - Microbiology
JF - Microbiology
SN - 1350-0872
IS - 5
ER -