Immunomodulating activities of polysaccharide fractions from dried safflower petals

Tetsuya Wakabayashi, Shigenari Hirokawa, Noriaki Yamauchi, Takao Kataoka, Je Tae Woo, Kazuo Nagai

研究成果: ジャーナルへの寄稿記事

24 引用 (Scopus)

抄録

In the course of screening for immunomodulators, we found a significant blastogenic activity specific for splenic B cells in the extracts of safflower (Carthamus tinctorius L.). Active fractions termed SF1 and SF2 were purified from dried petals of safflower by boiling water extraction, ethanol precipitation and Sepharose CL-2B column chromatography. The elution profiles of the gel filtration indicated that the molecular weight of SF1 and SF2 was estimated to be more than 100 kD. Major components of SF1 and SF2 seem to be polysaccharides, and structural analysis of alditol acetate derivatives by GC-MS revealed some differences between SF1 and SF2 in the sugar component. Biological activities of SF1 and SF2 on B cells and macrophages were examined in comparison with lipopolysaccharides (LPS). SF1 and SF2 induced both the proliferation and the IgM production of B cells to the equivalent level as those induced by LPS. In macrophages, SF1 and SF2 effectively stimulated the production of NO. However, SF1 stimulated the production of IL-1, IL-6, and TNF as much as LPS, while SF2 induced them only weakly or not at all. Thus, these results suggest that SF1 and SF2 activate B cells and macrophages in different mechanisms.

元の言語英語
ページ(範囲)205-211
ページ数7
ジャーナルCytotechnology
25
発行部数1-3
出版物ステータス出版済み - 12 1 1997

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Carthamus tinctorius
Polysaccharides
Macrophages
B-Lymphocytes
Cells
Lipopolysaccharides
Sugar Alcohols
Column chromatography
Immunologic Factors
Bioactivity
Cell Extracts
Interleukin-1
Structural analysis
Sugars
Sepharose
Boiling liquids
Gel Chromatography
Immunoglobulin M
Chromatography
Interleukin-6

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Bioengineering
  • Biomedical Engineering
  • Clinical Biochemistry
  • Cell Biology

これを引用

Wakabayashi, T., Hirokawa, S., Yamauchi, N., Kataoka, T., Woo, J. T., & Nagai, K. (1997). Immunomodulating activities of polysaccharide fractions from dried safflower petals. Cytotechnology, 25(1-3), 205-211.

Immunomodulating activities of polysaccharide fractions from dried safflower petals. / Wakabayashi, Tetsuya; Hirokawa, Shigenari; Yamauchi, Noriaki; Kataoka, Takao; Woo, Je Tae; Nagai, Kazuo.

:: Cytotechnology, 巻 25, 番号 1-3, 01.12.1997, p. 205-211.

研究成果: ジャーナルへの寄稿記事

Wakabayashi, T, Hirokawa, S, Yamauchi, N, Kataoka, T, Woo, JT & Nagai, K 1997, 'Immunomodulating activities of polysaccharide fractions from dried safflower petals', Cytotechnology, 巻. 25, 番号 1-3, pp. 205-211.
Wakabayashi T, Hirokawa S, Yamauchi N, Kataoka T, Woo JT, Nagai K. Immunomodulating activities of polysaccharide fractions from dried safflower petals. Cytotechnology. 1997 12 1;25(1-3):205-211.
Wakabayashi, Tetsuya ; Hirokawa, Shigenari ; Yamauchi, Noriaki ; Kataoka, Takao ; Woo, Je Tae ; Nagai, Kazuo. / Immunomodulating activities of polysaccharide fractions from dried safflower petals. :: Cytotechnology. 1997 ; 巻 25, 番号 1-3. pp. 205-211.
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