Immunophenotypic and functional characterization of CD33+CD34+ cells in human cord blood of preterm neonates

Cheng Hao Jin, Hidetoshi Takada, Akihiko Nomura, Yasushi Takahata, Hideki Nakayama, Makoto Kajiwara, Hitoo Nakano, Toshiro Hara

研究成果: ジャーナルへの寄稿記事

14 引用 (Scopus)

抜粋

Objective To characterize CD33+CD34+ cells, a major population in human cord blood (CB) CD34+ cells of preterm neonates. Materials and methods The proportion of CD33+ cells was analyzed on CB CD34+ cells from preterm and full-term neonates. CD33+CD34+ cells were purified by cell sorting and analyzed on their clonogenic activity, proliferative activity in short-time liquid suspension culture, and GATA-2 mRNA expression by RT-PCR and Southern blot. Results The absolute numbers and proportion of CD34+ cells in mononuclear cells inversely correlated with gestational age. CD33 was expressed on a majority of CB CD34+ cells of preterm neonates but on only a minor population of them in full-term neonates. In addition, CD33 was dominantly expressed on CD38-CD34+ cells or CD117(low)CD34+ cells in CB of preterm neonates. CD33+CD34+ cells of preterm cord blood had high proliferative and reproducible potentials compared with CD33-CD34+ cells. CD33+CD34+ cells as well as CD33-CD34+ cells from preterm CB highly expressed GATA-2, in contrast to those from BM. Conclusions These results suggest that CD33+CD34+ cells, which are a major population in CB CD34+ cells of preterm neonates, do not simply represent relatively mature myeloid lineage hematopoietic progenitor cells as those in adult BM CD34+ cells, and may contain hematopoietic stem cells or primitive progenitor cells as in fetal liver. (C) 2000 International Society for Experimental Hematology.

元の言語英語
ページ(範囲)1174-1180
ページ数7
ジャーナルExperimental Hematology
28
発行部数10
DOI
出版物ステータス出版済み - 10 1 2000

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Hematology
  • Genetics
  • Cell Biology
  • Cancer Research

フィンガープリント Immunophenotypic and functional characterization of CD33<sup>+</sup>CD34<sup>+</sup> cells in human cord blood of preterm neonates' の研究トピックを掘り下げます。これらはともに一意のフィンガープリントを構成します。

  • これを引用

    Jin, C. H., Takada, H., Nomura, A., Takahata, Y., Nakayama, H., Kajiwara, M., Nakano, H., & Hara, T. (2000). Immunophenotypic and functional characterization of CD33+CD34+ cells in human cord blood of preterm neonates. Experimental Hematology, 28(10), 1174-1180. https://doi.org/10.1016/S0301-472X(00)00519-1