Impact of a defective RNA 3 from cucumber mosaic virus on helper virus infection dynamics

Minoru Takeshita, Yuka Matsuo, Masashi Suzuki, Naruto Furuya, Kenichi Tsuchiya, Yoichi Takanami

研究成果: Contribution to journalArticle査読

4 被引用数 (Scopus)

抄録

D RNA 3Yα (D3Yα), a defective (D) RNA 3 derived from the Y strain of cucumber mosaic virus (CMV-Y), was further characterized in combination with different helper viruses in the genus Cucumovirus. Interestingly, Nicotiana benthamiana plants inoculated with CMV-D8 and D3Yα developed systemic symptoms which were different from those induced by CMV-D8. To elucidate the potential effects of D RNA 3 on virus infection on the basis of the original combination of CMV-Y and D3Yα, a point mutation was made in the coat protein gene, which determined symptoms, of either CMV-Y RNA 3 (Y3) or D3Yα. Symptoms induced on N. benthamiana and N. tabacum plants, and semi-quantitative RT-PCR revealed that the ratio of RNA 3 to D RNA 3 was associated with the differences of symptoms in the leaf tissues. Furthermore, analysis of in situ hybridization suggested that there were spatial effects between coat proteins of Y3 and D3Yα in the infected leaves.

本文言語英語
ページ(範囲)59-65
ページ数7
ジャーナルVirology
389
1-2
DOI
出版ステータス出版済み - 2009

All Science Journal Classification (ASJC) codes

  • Virology

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