Impaired chemotaxis and cell adhesion due to decrease in several cell-surface receptors in cathepsin E-deficient macrophages

Takayuki Tsukuba, Michiyo Yanagawa, Kuniaki Okamoto, Yoshiko Okamoto, Yoshiyuki Yasuda, Keiichi Nakayama, Tomoko Kadowaki, Kenji Yamamoto

研究成果: ジャーナルへの寄稿記事

18 引用 (Scopus)

抄録

Cathepsin E is an endo-lysosomal aspartic proteinase exclusively present in immune system cells. Previous studies have shown that cathepsin E-deficient (CatE /) mice display aberrant immune responses such as atopic dermatitis and higher susceptibility to bacterial infection. However, the mechanisms underlying abnormal immune responses induced by cathepsin E deficiency are still unclear. In this study, we found that the cell-surface levels of chemotactic receptors, including chemokine receptor (CCR)-2 and N-formyl peptide receptors (FPRs), were clearly diminished in CatE /macrophages compared with those in wild-type cells. Consistently, chemotaxis of CatE /macrophages to MCP-1 and N-formyl-methionyl- leucyl-phenylalanine was also decreased. Similar to the chemotactic receptors, the surface expressions of the adhesion receptors CD18 (integrin β2) and CD 29 (integrin β1) in CatE / macrophages were significantly decreased, thereby reducing cell attachment of CatE / macrophages. These results indicate that the defects in chemotaxis and cell adhesion are likely to be involved in the imperfect function of CatE /macrophages.

元の言語英語
ページ(範囲)565-573
ページ数9
ジャーナルJournal of biochemistry
145
発行部数5
DOI
出版物ステータス出版済み - 5 1 2009

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Cathepsin E
Macrophages
Cell adhesion
Cell Surface Receptors
Chemotaxis
Cell Adhesion
Integrins
Formyl Peptide Receptor
Aspartic Acid Proteases
N-Formylmethionine Leucyl-Phenylalanine
Chemokine Receptors
Immune system
Atopic Dermatitis
Bacterial Infections
Immune System

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology

これを引用

Impaired chemotaxis and cell adhesion due to decrease in several cell-surface receptors in cathepsin E-deficient macrophages. / Tsukuba, Takayuki; Yanagawa, Michiyo; Okamoto, Kuniaki; Okamoto, Yoshiko; Yasuda, Yoshiyuki; Nakayama, Keiichi; Kadowaki, Tomoko; Yamamoto, Kenji.

:: Journal of biochemistry, 巻 145, 番号 5, 01.05.2009, p. 565-573.

研究成果: ジャーナルへの寄稿記事

Tsukuba, T, Yanagawa, M, Okamoto, K, Okamoto, Y, Yasuda, Y, Nakayama, K, Kadowaki, T & Yamamoto, K 2009, 'Impaired chemotaxis and cell adhesion due to decrease in several cell-surface receptors in cathepsin E-deficient macrophages', Journal of biochemistry, 巻. 145, 番号 5, pp. 565-573. https://doi.org/10.1093/jb/mvp016
Tsukuba, Takayuki ; Yanagawa, Michiyo ; Okamoto, Kuniaki ; Okamoto, Yoshiko ; Yasuda, Yoshiyuki ; Nakayama, Keiichi ; Kadowaki, Tomoko ; Yamamoto, Kenji. / Impaired chemotaxis and cell adhesion due to decrease in several cell-surface receptors in cathepsin E-deficient macrophages. :: Journal of biochemistry. 2009 ; 巻 145, 番号 5. pp. 565-573.
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abstract = "Cathepsin E is an endo-lysosomal aspartic proteinase exclusively present in immune system cells. Previous studies have shown that cathepsin E-deficient (CatE /) mice display aberrant immune responses such as atopic dermatitis and higher susceptibility to bacterial infection. However, the mechanisms underlying abnormal immune responses induced by cathepsin E deficiency are still unclear. In this study, we found that the cell-surface levels of chemotactic receptors, including chemokine receptor (CCR)-2 and N-formyl peptide receptors (FPRs), were clearly diminished in CatE /macrophages compared with those in wild-type cells. Consistently, chemotaxis of CatE /macrophages to MCP-1 and N-formyl-methionyl- leucyl-phenylalanine was also decreased. Similar to the chemotactic receptors, the surface expressions of the adhesion receptors CD18 (integrin β2) and CD 29 (integrin β1) in CatE / macrophages were significantly decreased, thereby reducing cell attachment of CatE / macrophages. These results indicate that the defects in chemotaxis and cell adhesion are likely to be involved in the imperfect function of CatE /macrophages.",
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AU - Yasuda, Yoshiyuki

AU - Nakayama, Keiichi

AU - Kadowaki, Tomoko

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