Impaired development of the cerebellum in transgenic mice expressing the immediate-early protein IE180 of pseudorabies virus

Satoshi Taharaguchi, Yasuhiro Kon, Saori Yoshino, Etsuro Ono

研究成果: ジャーナルへの寄稿記事

16 引用 (Scopus)

抄録

Pseudorabies virus (PRV) infection in animals other than its natural host almost always gives rise to fatal diseases in the central nervous system as a result of infection of peripheral neurons and subsequently to the brain. PRV immediate-early protein (IE180) activates transcription of the PRV early and late genes, and other viral and cellular genes, and represses its own transcription. To examine specific effects of IE180 in neuropathogenicity, we have generated four transgenic mouse lines expressing IE180 in a tetracycline-regulated system. In the transgenic mouse lines, cerebellar symptoms such as ataxic gait, tremor and motor discoordination were observed. Histopathology of the cerebella in the transgenic mouse lines showing severe symptoms was remarkable for a failure of layer formation and a reduction in cerebellar size. These findings suggest that IE180 affects the cascade of gene expression for development of the murine cerebellum, resulting in the impairment of the cerebellar development and differentiation.

元の言語英語
ページ(範囲)243-254
ページ数12
ジャーナルVirology
307
発行部数2
DOI
出版物ステータス出版済み - 3 15 2003

Fingerprint

Cerebellum
Transgenic Mice
Suid Herpesvirus 1
Viral Genes
Central Nervous System Diseases
Tremor
Virus Diseases
Tetracycline
Gait
Gene Expression
Neurons
Brain
Infection
Genes
pseudorabies virus immediate early proteins

All Science Journal Classification (ASJC) codes

  • Virology

これを引用

Impaired development of the cerebellum in transgenic mice expressing the immediate-early protein IE180 of pseudorabies virus. / Taharaguchi, Satoshi; Kon, Yasuhiro; Yoshino, Saori; Ono, Etsuro.

:: Virology, 巻 307, 番号 2, 15.03.2003, p. 243-254.

研究成果: ジャーナルへの寄稿記事

Taharaguchi, Satoshi ; Kon, Yasuhiro ; Yoshino, Saori ; Ono, Etsuro. / Impaired development of the cerebellum in transgenic mice expressing the immediate-early protein IE180 of pseudorabies virus. :: Virology. 2003 ; 巻 307, 番号 2. pp. 243-254.
@article{3c893bea42314b76836c4997bf52c388,
title = "Impaired development of the cerebellum in transgenic mice expressing the immediate-early protein IE180 of pseudorabies virus",
abstract = "Pseudorabies virus (PRV) infection in animals other than its natural host almost always gives rise to fatal diseases in the central nervous system as a result of infection of peripheral neurons and subsequently to the brain. PRV immediate-early protein (IE180) activates transcription of the PRV early and late genes, and other viral and cellular genes, and represses its own transcription. To examine specific effects of IE180 in neuropathogenicity, we have generated four transgenic mouse lines expressing IE180 in a tetracycline-regulated system. In the transgenic mouse lines, cerebellar symptoms such as ataxic gait, tremor and motor discoordination were observed. Histopathology of the cerebella in the transgenic mouse lines showing severe symptoms was remarkable for a failure of layer formation and a reduction in cerebellar size. These findings suggest that IE180 affects the cascade of gene expression for development of the murine cerebellum, resulting in the impairment of the cerebellar development and differentiation.",
author = "Satoshi Taharaguchi and Yasuhiro Kon and Saori Yoshino and Etsuro Ono",
year = "2003",
month = "3",
day = "15",
doi = "10.1016/S0042-6822(02)00062-4",
language = "English",
volume = "307",
pages = "243--254",
journal = "Virology",
issn = "0042-6822",
publisher = "Academic Press Inc.",
number = "2",

}

TY - JOUR

T1 - Impaired development of the cerebellum in transgenic mice expressing the immediate-early protein IE180 of pseudorabies virus

AU - Taharaguchi, Satoshi

AU - Kon, Yasuhiro

AU - Yoshino, Saori

AU - Ono, Etsuro

PY - 2003/3/15

Y1 - 2003/3/15

N2 - Pseudorabies virus (PRV) infection in animals other than its natural host almost always gives rise to fatal diseases in the central nervous system as a result of infection of peripheral neurons and subsequently to the brain. PRV immediate-early protein (IE180) activates transcription of the PRV early and late genes, and other viral and cellular genes, and represses its own transcription. To examine specific effects of IE180 in neuropathogenicity, we have generated four transgenic mouse lines expressing IE180 in a tetracycline-regulated system. In the transgenic mouse lines, cerebellar symptoms such as ataxic gait, tremor and motor discoordination were observed. Histopathology of the cerebella in the transgenic mouse lines showing severe symptoms was remarkable for a failure of layer formation and a reduction in cerebellar size. These findings suggest that IE180 affects the cascade of gene expression for development of the murine cerebellum, resulting in the impairment of the cerebellar development and differentiation.

AB - Pseudorabies virus (PRV) infection in animals other than its natural host almost always gives rise to fatal diseases in the central nervous system as a result of infection of peripheral neurons and subsequently to the brain. PRV immediate-early protein (IE180) activates transcription of the PRV early and late genes, and other viral and cellular genes, and represses its own transcription. To examine specific effects of IE180 in neuropathogenicity, we have generated four transgenic mouse lines expressing IE180 in a tetracycline-regulated system. In the transgenic mouse lines, cerebellar symptoms such as ataxic gait, tremor and motor discoordination were observed. Histopathology of the cerebella in the transgenic mouse lines showing severe symptoms was remarkable for a failure of layer formation and a reduction in cerebellar size. These findings suggest that IE180 affects the cascade of gene expression for development of the murine cerebellum, resulting in the impairment of the cerebellar development and differentiation.

UR - http://www.scopus.com/inward/record.url?scp=0345268791&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0345268791&partnerID=8YFLogxK

U2 - 10.1016/S0042-6822(02)00062-4

DO - 10.1016/S0042-6822(02)00062-4

M3 - Article

C2 - 12667794

AN - SCOPUS:0345268791

VL - 307

SP - 243

EP - 254

JO - Virology

JF - Virology

SN - 0042-6822

IS - 2

ER -