抄録
Pseudorabies virus (PRV) infection in animals other than its natural host almost always gives rise to fatal diseases in the central nervous system as a result of infection of peripheral neurons and subsequently to the brain. PRV immediate-early protein (IE180) activates transcription of the PRV early and late genes, and other viral and cellular genes, and represses its own transcription. To examine specific effects of IE180 in neuropathogenicity, we have generated four transgenic mouse lines expressing IE180 in a tetracycline-regulated system. In the transgenic mouse lines, cerebellar symptoms such as ataxic gait, tremor and motor discoordination were observed. Histopathology of the cerebella in the transgenic mouse lines showing severe symptoms was remarkable for a failure of layer formation and a reduction in cerebellar size. These findings suggest that IE180 affects the cascade of gene expression for development of the murine cerebellum, resulting in the impairment of the cerebellar development and differentiation.
| 元の言語 | 英語 |
|---|---|
| ページ（範囲） | 243-254 |
| ページ数 | 12 |
| ジャーナル | Virology |
| 巻 | 307 |
| 発行部数 | 2 |
| DOI | |
| 出版物ステータス | 出版済み - 3 15 2003 |
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All Science Journal Classification (ASJC) codes
- Virology
これを引用
Impaired development of the cerebellum in transgenic mice expressing the immediate-early protein IE180 of pseudorabies virus. / Taharaguchi, Satoshi; Kon, Yasuhiro; Yoshino, Saori; Ono, Etsuro.
：: Virology, 巻 307, 番号 2, 15.03.2003, p. 243-254.研究成果: ジャーナルへの寄稿 › 記事
}
TY - JOUR
T1 - Impaired development of the cerebellum in transgenic mice expressing the immediate-early protein IE180 of pseudorabies virus
AU - Taharaguchi, Satoshi
AU - Kon, Yasuhiro
AU - Yoshino, Saori
AU - Ono, Etsuro
PY - 2003/3/15
Y1 - 2003/3/15
N2 - Pseudorabies virus (PRV) infection in animals other than its natural host almost always gives rise to fatal diseases in the central nervous system as a result of infection of peripheral neurons and subsequently to the brain. PRV immediate-early protein (IE180) activates transcription of the PRV early and late genes, and other viral and cellular genes, and represses its own transcription. To examine specific effects of IE180 in neuropathogenicity, we have generated four transgenic mouse lines expressing IE180 in a tetracycline-regulated system. In the transgenic mouse lines, cerebellar symptoms such as ataxic gait, tremor and motor discoordination were observed. Histopathology of the cerebella in the transgenic mouse lines showing severe symptoms was remarkable for a failure of layer formation and a reduction in cerebellar size. These findings suggest that IE180 affects the cascade of gene expression for development of the murine cerebellum, resulting in the impairment of the cerebellar development and differentiation.
AB - Pseudorabies virus (PRV) infection in animals other than its natural host almost always gives rise to fatal diseases in the central nervous system as a result of infection of peripheral neurons and subsequently to the brain. PRV immediate-early protein (IE180) activates transcription of the PRV early and late genes, and other viral and cellular genes, and represses its own transcription. To examine specific effects of IE180 in neuropathogenicity, we have generated four transgenic mouse lines expressing IE180 in a tetracycline-regulated system. In the transgenic mouse lines, cerebellar symptoms such as ataxic gait, tremor and motor discoordination were observed. Histopathology of the cerebella in the transgenic mouse lines showing severe symptoms was remarkable for a failure of layer formation and a reduction in cerebellar size. These findings suggest that IE180 affects the cascade of gene expression for development of the murine cerebellum, resulting in the impairment of the cerebellar development and differentiation.
UR - http://www.scopus.com/inward/record.url?scp=0345268791&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0345268791&partnerID=8YFLogxK
U2 - 10.1016/S0042-6822(02)00062-4
DO - 10.1016/S0042-6822(02)00062-4
M3 - Article
C2 - 12667794
AN - SCOPUS:0345268791
VL - 307
SP - 243
EP - 254
JO - Virology
JF - Virology
SN - 0042-6822
IS - 2
ER -