Impairment of cardiomyogenesis in embryonic stem cells lacking scaffold protein JSAP1

Tokiharu Sato, Kyoko Hidaka, Asuka Iwanaga, Michihiko Ito, Masahide Asano, Yusaku Nakabeppu, Takayuki Morisaki, Katsuji Yoshioka

研究成果: Contribution to journalArticle査読

10 被引用数 (Scopus)

抄録

We previously reported that c-Jun NH2-terminal kinase (JNK)/stress-activated protein kinase-associated protein 1 (JSAP1), a scaffold protein for JNK signaling, is important in embryonic stem (ES) cells during neurogenesis. In that study, we also observed the altered expression of mesodermal marker genes, which indicated that JSAP1 is involved in the differentiation of mesodermal lineages. Here, we investigated the function of JSAP1 in cardiomyocyte development using JSAP1-null ES cells, and found that cardiomyogenesis was impaired in the JSAP1-null mutant. The JSAP1 deficiency resulted in lower gene expression of the cardiac transcription factor Nkx2.5 and contractile proteins. In contrast, the mutant showed a significantly higher expression of mesoderm-related markers other than those of the cardiomyocyte lineage. Together, these results suggest that JSAP1 may be important for the differentiation of the mesodermal lineages, functioning as a positive factor for cardiomyocyte differentiation, and as an inhibitory factor for differentiation into other lineages.

本文言語英語
ページ(範囲)1152-1157
ページ数6
ジャーナルBiochemical and Biophysical Research Communications
338
2
DOI
出版ステータス出版済み - 12 16 2005

All Science Journal Classification (ASJC) codes

  • 生物理学
  • 生化学
  • 分子生物学
  • 細胞生物学

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