Impairment of Lhca4, a subunit of LHCI, causes high accumulation of chlorophyll and the stay-green phenotype in rice

Hiroshi Yamatani, Kaori Kohzuma, Michiharu Nakano, Tsuneaki Takami, Yusuke Kato, Yoriko Hayashi, Yuki Monden, Yutaka Okumoto, Tomoko Abe, Toshihiro Kumamaru, Ayumi Tanaka, Wataru Sakamoto, Makoto Kusaba

研究成果: ジャーナルへの寄稿記事

4 引用 (Scopus)

抄録

Chlorophyll is an essential molecule for acquiring light energy during photosynthesis. Mutations that result in chlorophyll retention during leaf senescence are called - € stay-green' mutants. One of the several types of stay-green mutants, Type E, accumulates high levels of chlorophyll in the pre-senescent leaves, resulting in delayed yellowing. We isolated delayed yellowing1-1 (dye1-1), a rice mutant whose yellowing is delayed in the field. dye1-1 accumulated more chlorophyll than the wild-type in the pre-senescent and senescent leaves, but did not retain leaf functionality in the - €- senescent green leaves', suggesting that dye1-1 is a Type E stay-green mutant. Positional cloning revealed that DYE1 encodes Lhca4, a subunit of the light-harvesting complex I (LHCI). In dye1-1, amino acid substitution occurs at the location of a highly conserved amino acid residue involved in pigment binding; indeed, a severely impaired structure of the PSI-LHCI super-complex in dye1-1 was observed in a blue native PAGE analysis. Nevertheless, the biomass and carbon assimilation rate of dye1-1 were comparable to those in the wild-type. Interestingly, Lhcb1, a trimeric LHCII protein, was highly accumulated in dye1-1, in the chlorophyll-protein complexes. The high accumulation of LHCII in the LHCI mutant dye1 suggests a novel functional interaction between LHCI and LHCII.

元の言語英語
ページ(範囲)1027-1035
ページ数9
ジャーナルJournal of Experimental Botany
69
発行部数5
DOI
出版物ステータス出版済み - 2 23 2018

Fingerprint

light harvesting complex
Chlorophyll
Phenotype
chlorophyll
Light
phenotype
rice
mutants
leaves
Chlorophyll Binding Proteins
Native Polyacrylamide Gel Electrophoresis
Photosynthesis
amino acid substitution
Amino Acid Substitution
Biomass
Organism Cloning
molecular cloning
Carbon
pigments
Oryza

All Science Journal Classification (ASJC) codes

  • Physiology
  • Plant Science

これを引用

Impairment of Lhca4, a subunit of LHCI, causes high accumulation of chlorophyll and the stay-green phenotype in rice. / Yamatani, Hiroshi; Kohzuma, Kaori; Nakano, Michiharu; Takami, Tsuneaki; Kato, Yusuke; Hayashi, Yoriko; Monden, Yuki; Okumoto, Yutaka; Abe, Tomoko; Kumamaru, Toshihiro; Tanaka, Ayumi; Sakamoto, Wataru; Kusaba, Makoto.

:: Journal of Experimental Botany, 巻 69, 番号 5, 23.02.2018, p. 1027-1035.

研究成果: ジャーナルへの寄稿記事

Yamatani, H, Kohzuma, K, Nakano, M, Takami, T, Kato, Y, Hayashi, Y, Monden, Y, Okumoto, Y, Abe, T, Kumamaru, T, Tanaka, A, Sakamoto, W & Kusaba, M 2018, 'Impairment of Lhca4, a subunit of LHCI, causes high accumulation of chlorophyll and the stay-green phenotype in rice', Journal of Experimental Botany, 巻. 69, 番号 5, pp. 1027-1035. https://doi.org/10.1093/jxb/erx468
Yamatani, Hiroshi ; Kohzuma, Kaori ; Nakano, Michiharu ; Takami, Tsuneaki ; Kato, Yusuke ; Hayashi, Yoriko ; Monden, Yuki ; Okumoto, Yutaka ; Abe, Tomoko ; Kumamaru, Toshihiro ; Tanaka, Ayumi ; Sakamoto, Wataru ; Kusaba, Makoto. / Impairment of Lhca4, a subunit of LHCI, causes high accumulation of chlorophyll and the stay-green phenotype in rice. :: Journal of Experimental Botany. 2018 ; 巻 69, 番号 5. pp. 1027-1035.
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abstract = "Chlorophyll is an essential molecule for acquiring light energy during photosynthesis. Mutations that result in chlorophyll retention during leaf senescence are called - € stay-green' mutants. One of the several types of stay-green mutants, Type E, accumulates high levels of chlorophyll in the pre-senescent leaves, resulting in delayed yellowing. We isolated delayed yellowing1-1 (dye1-1), a rice mutant whose yellowing is delayed in the field. dye1-1 accumulated more chlorophyll than the wild-type in the pre-senescent and senescent leaves, but did not retain leaf functionality in the - €- senescent green leaves', suggesting that dye1-1 is a Type E stay-green mutant. Positional cloning revealed that DYE1 encodes Lhca4, a subunit of the light-harvesting complex I (LHCI). In dye1-1, amino acid substitution occurs at the location of a highly conserved amino acid residue involved in pigment binding; indeed, a severely impaired structure of the PSI-LHCI super-complex in dye1-1 was observed in a blue native PAGE analysis. Nevertheless, the biomass and carbon assimilation rate of dye1-1 were comparable to those in the wild-type. Interestingly, Lhcb1, a trimeric LHCII protein, was highly accumulated in dye1-1, in the chlorophyll-protein complexes. The high accumulation of LHCII in the LHCI mutant dye1 suggests a novel functional interaction between LHCI and LHCII.",
author = "Hiroshi Yamatani and Kaori Kohzuma and Michiharu Nakano and Tsuneaki Takami and Yusuke Kato and Yoriko Hayashi and Yuki Monden and Yutaka Okumoto and Tomoko Abe and Toshihiro Kumamaru and Ayumi Tanaka and Wataru Sakamoto and Makoto Kusaba",
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AU - Yamatani, Hiroshi

AU - Kohzuma, Kaori

AU - Nakano, Michiharu

AU - Takami, Tsuneaki

AU - Kato, Yusuke

AU - Hayashi, Yoriko

AU - Monden, Yuki

AU - Okumoto, Yutaka

AU - Abe, Tomoko

AU - Kumamaru, Toshihiro

AU - Tanaka, Ayumi

AU - Sakamoto, Wataru

AU - Kusaba, Makoto

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