Implantation of a new porous gelatin-siloxane hybrid into a brain lesion as a potential scaffold for tissue regeneration

Kentaro Deguchi, Kanji Tsuru, Takeshi Hayashi, Mikiro Takaishi, Mitsuyuki Nagahara, Shoko Nagotani, Yoshihide Sehara, Guang Jin, Han Zhe Zhang, Satoshi Hayakawa, Mikio Shoji, Masahiro Miyazaki, Akiyoshi Osaka, Nam Ho Huh, Koji Abe

研究成果: ジャーナルへの寄稿学術誌査読

60 被引用数 (Scopus)

抄録

For brain tissue regeneration, any scaffold for migrated or transplanted stem cells with supportive angiogenesis is important once necrotic brain tissue has formed a cavity after injury such as cerebral ischemia. In this study, a new porous gelatin-siloxane hybrid derived from the integration of gelatin and 3-(glycidoxypropyl) trimethoxysilane was implanted as a three-dimensional scaffold into a defect of the cerebral cortex. The porous hybrid implanted into the lesion remained at the same site for 60 days, kept integrity of the brain shape, and attached well to the surrounding brain tissues. Marginal cavities of the scaffolds were occupied by newly formed tissue in the brain, where newly produced vascular endothelial, astroglial, and microglial cells were found with bromodeoxyuridine double positivity, and the numbers of those cells were dose-dependently increased with the addition of basic fibroblast growth factor (bFGF) and epidermal growth factor (EGF). Extension of dendrites was also found from the surrounding cerebral cortex to the newly formed tissue, especially with the addition of bFGF and EGF. The present study showed that a new porous gelatin-siloxane hybrid had biocompatibility after implantation into a lesion of the central nervous system, and thus provided a potential scaffold for cell migration, angiogenesis and dendrite elongation with dose-dependent effects of additive bFGF and EGF.

本文言語英語
ページ(範囲)1263-1273
ページ数11
ジャーナルJournal of Cerebral Blood Flow and Metabolism
26
10
DOI
出版ステータス出版済み - 10月 18 2006
外部発表はい

!!!All Science Journal Classification (ASJC) codes

  • 神経学
  • 臨床神経学
  • 循環器および心血管医学

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