Improved fluorogenic assay for rapid detection of Vibrio parahaemolyticus in foods

Takahisa Miyamoto, H. Miwa, S. Hatano

研究成果: ジャーナルへの寄稿記事

13 引用 (Scopus)

抄録

An improved fluorogenic assay for the rapid detection of Vibrio parahaemolyticus was developed. In the improved assay, the enrichment of V. parahaemolyticus was carried out in arabinose-glucuronate medium (0.5% arabinose, 0.25% glucuronate, 0.1% polypeptone, 0.1% yeast extract, 0.1% ammonium sulfate, 2% NaCl, 2 μg of polymyxin B sulfate per ml, pH 8.5) at 37°C. After the cultivation, the trypsinlike activity of the bacteria was measured by fluorescence with the fluorogenic substrate benzoyl-L-arginine-7-aminomethylcoumarin. Even in the presence of 3 x 105 cells of Vibrio alginolyticus, 20 cells of V. parahaemolyticus were clearly detected after a 6-h enrichment cultivation by the assay. Fifty contaminated samples of 14 seafoods were examined for V. parahaemolyticus by the fluorogenic assay after enrichment cultivation for 6 or 8 h. The results were then compared with those obtained by the conventional bromothymol blue Teepol agar assay and the most-probable-number method. There was a linear relationship between trypsinlike activity measured by the assay and the number of V. parahaemolyticus cells in seafood as determined by the bromothymol blue Teepol agar and most-probable-number methods. Correlation coefficients were 0.95 and 0.93 after a 6-h cultivation and an 8-h cultivation, respectively. The presence of 10 cells of V. parahaemolyticus per gram of seafood sample was detected after a 10-h total detection time by the fluorogenic assay.

元の言語英語
ページ(範囲)1480-1484
ページ数5
ジャーナルApplied and Environmental Microbiology
56
発行部数5
出版物ステータス出版済み - 1990

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Vibrio parahaemolyticus
assay
Food
Seafood
food
Bromthymol Blue
assays
seafood
seafoods
Glucuronic Acid
Arabinose
Agar
arabinose
agar
Vibrio alginolyticus
Polymyxin B
cells
polymyxin B
Ammonium Sulfate
Fluorescent Dyes

All Science Journal Classification (ASJC) codes

  • Environmental Science(all)
  • Biotechnology
  • Microbiology

これを引用

Improved fluorogenic assay for rapid detection of Vibrio parahaemolyticus in foods. / Miyamoto, Takahisa; Miwa, H.; Hatano, S.

:: Applied and Environmental Microbiology, 巻 56, 番号 5, 1990, p. 1480-1484.

研究成果: ジャーナルへの寄稿記事

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abstract = "An improved fluorogenic assay for the rapid detection of Vibrio parahaemolyticus was developed. In the improved assay, the enrichment of V. parahaemolyticus was carried out in arabinose-glucuronate medium (0.5{\%} arabinose, 0.25{\%} glucuronate, 0.1{\%} polypeptone, 0.1{\%} yeast extract, 0.1{\%} ammonium sulfate, 2{\%} NaCl, 2 μg of polymyxin B sulfate per ml, pH 8.5) at 37°C. After the cultivation, the trypsinlike activity of the bacteria was measured by fluorescence with the fluorogenic substrate benzoyl-L-arginine-7-aminomethylcoumarin. Even in the presence of 3 x 105 cells of Vibrio alginolyticus, 20 cells of V. parahaemolyticus were clearly detected after a 6-h enrichment cultivation by the assay. Fifty contaminated samples of 14 seafoods were examined for V. parahaemolyticus by the fluorogenic assay after enrichment cultivation for 6 or 8 h. The results were then compared with those obtained by the conventional bromothymol blue Teepol agar assay and the most-probable-number method. There was a linear relationship between trypsinlike activity measured by the assay and the number of V. parahaemolyticus cells in seafood as determined by the bromothymol blue Teepol agar and most-probable-number methods. Correlation coefficients were 0.95 and 0.93 after a 6-h cultivation and an 8-h cultivation, respectively. The presence of 10 cells of V. parahaemolyticus per gram of seafood sample was detected after a 10-h total detection time by the fluorogenic assay.",
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