Improvement of isopropanol production by metabolically engineered Escherichia coli using gas stripping

Kentaro Inokuma, James C. Liao, Masahiro Okamoto, Taizo Hanai

研究成果: ジャーナルへの寄稿記事

99 引用 (Scopus)

抄録

To improve isopropanol production by metabolically engineered Escherichia coli strain TA76, the optimization of fermentation conditions and isopropanol removal by gas stripping were performed. Isopropanol is one of the simplest secondary alcohols, and it can be dehydrated to yield propylene, which is currently derived from petroleum as a monomer for making polypropylene. Initially, using a pH-controlled fed-batch culture with the intermittent addition of glucose, strain TA76 produced 667. mM (40.1. g/L) of isopropanol after 60. h, representing 73.2% (mol isopropanol/mol glucose) of the theoretical maximum yield. Because the accumulation of isopropanol drastically reduced production yields, a gas stripping recovery method was incorporated into the fed-batch culture system. Using this approach, strain TA76 produced 2378. mM (143. g/L) of isopropanol after 240. h with a yield of 67.4% (mol/mol). To our knowledge, this titer represents the highest level of isopropanol production by E. coli to date and suggests that strain TA76 has a great potential for commercial fermentative isopropanol production.

元の言語英語
ページ(範囲)696-701
ページ数6
ジャーナルJournal of Bioscience and Bioengineering
110
発行部数6
DOI
出版物ステータス出版済み - 12 1 2010

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2-Propanol
Escherichia coli
Gases
Glucose
Batch Cell Culture Techniques
Fermentation
Propylene
Polypropylenes
Alcohols
Crude oil
Monomers
Recovery
Petroleum

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology

これを引用

Improvement of isopropanol production by metabolically engineered Escherichia coli using gas stripping. / Inokuma, Kentaro; Liao, James C.; Okamoto, Masahiro; Hanai, Taizo.

:: Journal of Bioscience and Bioengineering, 巻 110, 番号 6, 01.12.2010, p. 696-701.

研究成果: ジャーナルへの寄稿記事

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abstract = "To improve isopropanol production by metabolically engineered Escherichia coli strain TA76, the optimization of fermentation conditions and isopropanol removal by gas stripping were performed. Isopropanol is one of the simplest secondary alcohols, and it can be dehydrated to yield propylene, which is currently derived from petroleum as a monomer for making polypropylene. Initially, using a pH-controlled fed-batch culture with the intermittent addition of glucose, strain TA76 produced 667. mM (40.1. g/L) of isopropanol after 60. h, representing 73.2{\%} (mol isopropanol/mol glucose) of the theoretical maximum yield. Because the accumulation of isopropanol drastically reduced production yields, a gas stripping recovery method was incorporated into the fed-batch culture system. Using this approach, strain TA76 produced 2378. mM (143. g/L) of isopropanol after 240. h with a yield of 67.4{\%} (mol/mol). To our knowledge, this titer represents the highest level of isopropanol production by E. coli to date and suggests that strain TA76 has a great potential for commercial fermentative isopropanol production.",
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