Improvement of Sensitivity and Reproducibility for Imaging of Endogenous Metabolites by Matrix-Assisted Laser Desorption/Ionization-Mass Spectrometry

Tomomi Morikawa-Ichinose, Yoshinori Fujimura, Fusa Murayama, Yuzo Yamazaki, Takushi Yamamoto, Hiroyuki Wariishi, Daisuke Miura

研究成果: ジャーナルへの寄稿記事

1 引用 (Scopus)

抄録

Matrix-assisted laser desorption/ionization (MALDI)-mass spectrometry imaging (MSI) is a powerful technique to visualize the distributions of biomolecules without any labeling. In MALDI-MSI experiments, the choice of matrix deposition method is important for acquiring favorable MSI data with high sensitivity and high reproducibility. Generally, manual or automated spray-coating and automated sublimation methods are used, but these methods have some drawbacks with respect to detection sensitivity, spatial resolution, and data reproducibility. Herein, we present an optimized matrix deposition method of sublimation coupled with recrystallization using 9-aminoacridine (9-AA) as a matrix capable of ionizing endogenous metabolites. The matrix recrystallization process after sublimation was optimized for the solvent concentration and reaction temperature for matrix–metabolite co-crystallization. This optimized method showed excellent reproducibility and spatial resolution compared to the automatic spray-coating method. Furthermore, the recrystallization step after sublimation remarkably improved the detectability of metabolites, including amino acids, nucleotide derivatives, and lipids, compared with the conventional sublimation method. To date, there have been no other reports of 9-AA-based sublimation combined with recrystallization. The present method provides an easy, sensitive, and reproducible matrix deposition method for MALDI-MSI of endogenous metabolites. [Figure not available: see fulltext.].

元の言語英語
ページ(範囲)1512-1520
ページ数9
ジャーナルJournal of the American Society for Mass Spectrometry
30
発行部数8
DOI
出版物ステータス出版済み - 8 15 2019

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Sublimation
Matrix-Assisted Laser Desorption-Ionization Mass Spectrometry
Metabolites
Ionization
Mass spectrometry
Desorption
Imaging techniques
Lasers
Crystallization
Aminacrine
Coatings
Biomolecules
Labeling
Nucleotides
Derivatives
Lipids
Amino Acids
Mass Spectrometry
Temperature
Experiments

All Science Journal Classification (ASJC) codes

  • Structural Biology
  • Spectroscopy

これを引用

Improvement of Sensitivity and Reproducibility for Imaging of Endogenous Metabolites by Matrix-Assisted Laser Desorption/Ionization-Mass Spectrometry. / Morikawa-Ichinose, Tomomi; Fujimura, Yoshinori; Murayama, Fusa; Yamazaki, Yuzo; Yamamoto, Takushi; Wariishi, Hiroyuki; Miura, Daisuke.

:: Journal of the American Society for Mass Spectrometry, 巻 30, 番号 8, 15.08.2019, p. 1512-1520.

研究成果: ジャーナルへの寄稿記事

Morikawa-Ichinose, Tomomi ; Fujimura, Yoshinori ; Murayama, Fusa ; Yamazaki, Yuzo ; Yamamoto, Takushi ; Wariishi, Hiroyuki ; Miura, Daisuke. / Improvement of Sensitivity and Reproducibility for Imaging of Endogenous Metabolites by Matrix-Assisted Laser Desorption/Ionization-Mass Spectrometry. :: Journal of the American Society for Mass Spectrometry. 2019 ; 巻 30, 番号 8. pp. 1512-1520.
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