Improvement of the cellular quality of cryopreserved bovine blastocysts accompanied by enhancement of the ATP-binding cassette sub-family B member 1 expression

Miyuki Mori, Shojiro Kasa, Yoshihiro Isozaki, Tsugumitsu Kamori, Shoichiro Yamaguchi, Shuji Ueda, Toshio Kuwano, Minako Eguchi, Keishiro Isayama, Shotaro Nishimura, Shoji Tabata, Nobuhiko Yamauchi, Masa aki Hattori

研究成果: ジャーナルへの寄稿記事

7 引用 (Scopus)

抄録

The ATP-binding cassette sub-family B member 1 (ABCB1) plays a critical role in maintaining the metabolic capability of cells as an efflux transporter that pumps xenobiotics out of cells. We investigated the effects of highly expressed ABCB1 on the development and viability of cryopreserved bovine embryos. The ABCB1 level in cultured bovine embryos was decreased during development to blastocyst-stage compared to germinal vesicle- and second metaphase-stage oocytes. When bovine embryos were cultured with forskolin and/or rifampicin, the ABCB1 level was significantly increased in blastocysts but embryo development was not significantly improved. After embryo cryopreservation, highly ABCB1-expressed blastocysts exhibited significant increases in viability and hatching rates. The high viability of the cryopreserved blastocysts was accompanied by a significant increase in cell proliferation during culture for 48. h. Thus, ABCB1 is expressed in bovine oocytes and embryos, and the cellular quality of bovine blastocysts is improved by the enhancement of ABCB1 expression.

元の言語英語
ページ(範囲)17-24
ページ数8
ジャーナルReproductive Toxicology
35
発行部数1
DOI
出版物ステータス出版済み - 1 1 2013

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P-Glycoprotein
Blastocyst
Quality Improvement
Embryonic Structures
Oocytes
Cryopreservation
Cell proliferation
Xenobiotics
Colforsin
Rifampin
Metaphase
Cell culture
Embryonic Development
Cell Proliferation
Pumps

All Science Journal Classification (ASJC) codes

  • Toxicology

これを引用

Improvement of the cellular quality of cryopreserved bovine blastocysts accompanied by enhancement of the ATP-binding cassette sub-family B member 1 expression. / Mori, Miyuki; Kasa, Shojiro; Isozaki, Yoshihiro; Kamori, Tsugumitsu; Yamaguchi, Shoichiro; Ueda, Shuji; Kuwano, Toshio; Eguchi, Minako; Isayama, Keishiro; Nishimura, Shotaro; Tabata, Shoji; Yamauchi, Nobuhiko; Hattori, Masa aki.

:: Reproductive Toxicology, 巻 35, 番号 1, 01.01.2013, p. 17-24.

研究成果: ジャーナルへの寄稿記事

Mori, Miyuki ; Kasa, Shojiro ; Isozaki, Yoshihiro ; Kamori, Tsugumitsu ; Yamaguchi, Shoichiro ; Ueda, Shuji ; Kuwano, Toshio ; Eguchi, Minako ; Isayama, Keishiro ; Nishimura, Shotaro ; Tabata, Shoji ; Yamauchi, Nobuhiko ; Hattori, Masa aki. / Improvement of the cellular quality of cryopreserved bovine blastocysts accompanied by enhancement of the ATP-binding cassette sub-family B member 1 expression. :: Reproductive Toxicology. 2013 ; 巻 35, 番号 1. pp. 17-24.
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